Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell RNA sequencing on PyMT mammary gland tumor cells

ABSTRACT: Single cell RNA sequencing on PyMT mammary gland tumor cells

PROVIDER: PRJNA639108 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single cell RNA sequencing on PyMT mammary gland tumor cells

Project description:We labeled PyMT control cells versus PyMT-GPx2 KD with GFP in vitro and then injected into mammary fat pad of mice for incubating 45 days. We then generated single cell suspension by FACS sorting from PyMT-control, GPx2 KD. 10X Genomics was used to make cDNA library. We then sequenced the samples with Illumina high throughput sequencing.

2022-01-13 | GSE152368 | GEO

Gene expression profiles of the mammary gland tumors from Nr1d1+/+;PyMT and Nr1d1-/-;PyMT mice

Project description:To investigate the role of NR1D1 in the progression of breast cancer, mammary gland tumor tissues were obtained from 14 weeks old FVB Nr1d1+/+;PyMT and Nr1d1-/-;PyMT mice and the gene expression patterns were analyzed by RNA-seq.

2023-06-27 | GSE222645 | GEO

PyMT 4 stages tumor progression microRNA sequencing

Project description:We sequenced mammary gland samples of MMTV-PyMT mouse from 4 stages (hyperplasia at week 6, adenoma/MIN at week 8, early carcinoma at week 10, and late carcinoma with lung metastasis at week 12) during tumor progression. We sacrificed 3 PyMT (FVB background) mice and 3 FVB control mice at each of the 4 stages, extracted small RNA and performed sequencing.

2016-06-10 | E-GEOD-83189 | biostudies-arrayexpress

Mammary gland tumor immunoinfiltrate analysis

Project description:Mammary gland tumor immunoinfiltrate analysis

| PRJNA634093 | ENA

Mammary gland tumor immunoinfiltrate analysis

Project description:Mammary gland tumor immunoinfiltrate analysis

| PRJNA634255 | ENA

Expression data of MMTV-PyMT mice mammary tumor with or without JAM-A

Project description:Junction Adhesion Molecule-A (JAM-A) is present on leukocytes and platelets where it promotes cell adhesion and motility. We are interested in an interaction between JAM-A and tumor progression/metastases. To address this point, we mated JAM-A-/- mice and mouse mammary tumor model MMTV-PyMT mice which, which express polyoma middle T antigen under the control of mouse mammary tumor virus. MMTV-PyMT mice show 100% penetration of mammary tumor and highly metastases to lung. MMTV-PyMT mice without JAM-A show less primary tumor progression, therefore JAM-A enhance primary tumor progression. Then we are addressing the molecular mechanism of this phenomenon by in vivo. Furthermore, we would like to examine JAM-A deficient MMTV tumor signature. Each 3 MMTV-PyMT JAm-A+/+ (JamA+) and 3 MMTV JAM-A-/- (JamA-) mammary tumor were resected at early stages of tumor development for RNA extraction and hybridization on Affymetrix microarrays.

2010-10-01 | E-GEOD-22555 | biostudies-arrayexpress

Single cell RNA sequencing on PyMT mammary gland primary tumor and lung metastases

Project description:Using scRNA-seq of the PyMT/GPx2 KD versus the PyMT control tumor model, we identified an overlap in cell clustering involving six luminal-like clusters and one basal/mesenchymal-like cluster (cluster 3). Similarly, scRNA-seq of lung metastases derived from the GPx2 KD tumor unraveled several luminal-like clusters and one mesenchymal-like cluster (M-cluster) which was highly homologous to primary tumor cluster 3.

2023-04-29 | GSE215394 | GEO

PyMT 4 stages tumor progression microRNA sequencing

2016-06-10 | GSE83189 | GEO

PyMT 4 stages tumor progression RNA-seq

2017-03-29 | GSE76772 | GEO

Breast tumor from Pik3caH1047R/+ mammary gland transduced with single or pooled lentivirus.

Project description:RNA-seq of breast tumor from Pik3caH1047R/+ mammary gland transduced with single or pooled lentivirus.

2018-11-18 | GSE122658 | GEO