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Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNAtag-seq]

ABSTRACT: Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNAtag-seq]

PROVIDER: PRJNA639521 | ENA |

REPOSITORIES: ENA

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Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator

Project description:Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator

| PRJNA639519 | ENA

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNA-Seq]

Project description:In the opportunistic pathogen Pseudomonas aeruginosa the alp system encodes a programmed cell death (PCD) pathway that is switched on in a subset of cells in response to DNA damage. Here we show that the central regulator of this pathway, AlpA, exerts its effects by acting as an antiterminator rather than a transcription activator. In particular, we present evidence that AlpA positively regulates the alpBCDE cell lysis genes, as well as genes in a second newly identified operon, by recognizing specific DNA sites within the promoter, then binding RNA polymerase directly and allowing it to bypass intrinsic terminators positioned downstream. We further show that the activity of AlpA is potentiated by the alarmone ppGpp, which is produced by the cell in response to stress. Through its responsiveness to ppGpp, AlpA can integrate environmental cues into the decision to execute a PCD pathway that is linked to the virulence of the organism.

2021-01-19 | GSE152484 | GEO

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [ChIP-Seq]

2021-01-19 | GSE152481 | GEO

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNAtag-seq]

2021-01-19 | GSE152480 | GEO

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [ChIP-Seq]

Project description:Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [ChIP-Seq]

| PRJNA639522 | ENA

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNA-Seq]

Project description:Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator [RNA-Seq]

| PRJNA639523 | ENA

Control of a programmed cell death pathway in Pseudomonas aeruginosa by a ppGpp-responsive antiterminator

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-01-19 | GSE152485 | GEO

Transcriptome analysis of programmed cell death during allorecognition in Cryphonectria parasitica

Project description:Transcriptome analysis of programmed cell death during allorecognition in Cryphonectria parasitica

| PRJNA666425 | ENA

Transcription profiling of E. coli cells during cell death programmed by a restriction-modification system

Project description:Genetically programmed deaths play important roles in the biology of unicellular prokaryotic cells. Some gene complexes force their maintenance on the host bacterial cells by killing cells that have lost them. This form of programmed death called post-segregational killing or genetic addiction is brought about by several Type II restriction-modification gene complexes, through restriction attack on the undermethylated chromosome, and underlie their behavior as selfish mobile elements. To learn the genetic steps to death, we examined how carriage and loss of PaeR7I restriction-modification gene complex affect host Escherichia coli cells through transcriptome and experimental analyses. The PaeR7I complex was on a temperature-sensitive plasmid so that the killing was induced by a temperature shift. We used microarrays to detail the global program of gene expression underlying cell death process mediated by PaeR7I restriction-modification system in E. coli. Experiment Overall Design: Post-segregational cell killing was induced by blocking replication of a temperature sensitive plasmid carrying PaeR7I RM gene complex by shifting up the cultivation temperature. At 30℃, the permissive temperature for the plasmid replication, growth as monitored by OD660, of MG1655/pTN9 (r+m+) was indistinguishable from those of MG1655/pTN11 (r-m+) and MG1655/pHSG415 (vector). Cell death was observed at least 4 h after the temperature shift only in MG1655/pTN9 (r+m+) as in the previous works; the increase in viable cell counts stopped and resulted in decrease of cell viability. To analyze global gene expression when cells went to death, we performed transcriptome analysis 0 h, 1h, 1 h 50 min, and 3h after the temperature shift. We used Affimetrix E. coli antisense genome array. Experiments were performed independently twice.

2009-07-04 | E-GEOD-7326 | biostudies-arrayexpress

Expression profiles during cell death programmed by a restriction-modification system in E. coli

2009-06-23 | GSE7326 | GEO

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