Project description:We generated genome-wide ChIP-seq binding profiles of BRD4 to analyze the role of YFV capsid lysine residues in the levels of BRD4 on chromatin

2020-11-10 | GSE151772 | GEO

Genome-wide BRD4 occupancy upon expression of YFV capsid in Huh-7 cells

Project description:Genome-wide BRD4 occupancy upon expression of YFV capsid in Huh-7 cells

| PRJNA637227 | ENA

Transcriptomic analysis of transfected Huh7 cells highlights capsid role in reprogramming cellular and metabolic processes during CHIKV infection

Project description:The purpose of this study is to compare the gene profiles of chikungunya virus (CHIKV) capsid transfected and CHIKV infected Huh7 cells to identify the genes differentially expressed in the presence of the capsid protein during CHIKV infection

2021-03-16 | GSE168910 | GEO

The yellow fever virus capsid protein is a potent suppressor of RNA silencing that binds double stranded RNA.

Project description:In this study, we describe a viral suppressor of RNA silencing encoded by the prototype flavivirus, yellow fever virus (YFV). We show that the YFV capsid protein inhibits RNA silencing in the mosquito Aedes aegypti by interfering with Dicer. These results suggest a molecular arms race between vector and pathogen underlies the continued existence of flaviviruses in nature.

2017-01-04 | GSE80691 | GEO

Expression data from the hepatitis E virus capsid protein

Project description:The hepatitis E virus (HEV), a non enveloped RNA virus, causes viral hepatitis. The viral open reading frame 2 (ORF2) protein represents the capsid protein of HEV which is known to cause endoplasmic reticulum stress in ORF2 expressing cells. The initiation of endoplasmic reticulum stress induced apoptosis mainly involves the transcriptional activation of pro-apoptotic gene CHOP which will further trigger the major apoptotic pathways. However, the activation of CHOP by ORF2 protein in this study does not induce apoptotic markers such as Bax translocation to mitochondria. We have used the Affymetrix microarray platform to screen the pro-apoptotic effects induced by the expression of ORF2 protein in human hepatic cell lines (Huh7). The Huh7 cells were transduced either with recombinant adenovirus encoding the HEV ORF2 (Ad-ORF2) or an adenovirus encoding the green fluorescent protein (Ad-GFP). The array results consistently showed an ORF2 specific induction of mRNA corresponding to the chaperones Hsp72, Hsp70B’ and co-chaperone Hsp40. These studies provide further mechanisms of the ER stress mediated pro apoptotic effects caused by the ORF2 protein and its potential role for the activation of anti-apoptotic activity of the host cell. We used microarray to screen the host genes were regulated by the expression of the hepatitis E virus capsid protein. Huh7 cells transduced with Ad-GFP (control) or with Ad-HEV ORF2.

2012-12-01 | E-GEOD-29061 | biostudies-arrayexpress

Transcriptome profiling data of THP-1 cells upon Huh7 cell-derived ectosomes incubation

Project description:Analysis of the transcriptome of THP-1 cells upon Huh7 cell-derived ectosomes incubation. Monocytic THP-1 cells were incubated with or without ectosomes (10 mg/ml) derived from scramble sequence transfection (Scramble Ecto) or PKM2 knocking down (PKM2 KD Ecto) Huh7 cells for 24 h. Results provide a novel insight into monocyte differentiation.

2022-06-08 | GSE132435 | GEO

Expression data from Rhesus immune cells responding to YFV

Project description:Immune response to infection involves the regulation of numerouse genes in numerous cell types. The number and type of genes that become differentially expressed in response to infection can result in very different pathophysiologic presentations and disease course. Wild type YFV and 17D despite having very few genetic differences result in very different disease outcomes in human and monkey hosts. We used microarrays to detail the global programme of gene expression of Rhesus macaque immune cells responding to both wild type and 17D strains of YFV. We identified distinct classes of gene expression as well as marked differences in differential gene expression between responses to 17D and wild type strains of YFV. Rhesus PBMC were isolated before and on day 3 of either vaccination with 17D or infection with wild type YFV with an n of 3 per group. Total RNA was extracted and hybridized on Affymetrix microarrays.

2014-10-22 | E-GEOD-51972 | biostudies-arrayexpress

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