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Gene expression profile of supernumerary type II neuroblasts transiently overexpressing Insb

ABSTRACT: Gene expression profile of supernumerary type II neuroblasts transiently overexpressing Insb

PROVIDER: PRJNA639873 | ENA |

REPOSITORIES: ENA

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Gene expression profile of supernumerary type II neuroblasts transiently overexpressing Insb

Project description:Genes that positively regulate neuroblast functional identity shoud be expressed in type II neuroblasts and become repidly downregulated in immature INPs in wild-type brains while genes that suppress neuroblast functional idnetity should be upregulated in immature INPs. The goal of this study is to identify genes involved in regulation of type II neuroblast functional identity.

2020-12-02 | GSE152636 | GEO

Transcriptomes of lineage-specific Drosophila neuroblasts profiled via genetic targeting and robotic sorting

Project description:A brain consists of numerous distinct neurons arising from a limited number of progenitors, called neuroblasts in Drosophila. Each neuroblast makes a specific neuronal lineage. To unravel the transcriptional networks that underlie the development of distinct neuroblast lineages, we marked and isolated lineage-specific neuroblasts for RNA sequencing. We labeled particular neuroblasts throughout neurogenesis by activating a conditional neuroblast driver in specific lineages using various intersection strategies. The targeted neuroblasts were efficiently recovered using a custom-built device for robotic single cell picking. Transcriptome analysis on the mushroom body, antennal lobe, and type II neuroblasts besides non-selective neuroblasts, neurons, and glia revealed a rich repertoire of transcription factors expressed among neuroblasts in diverse patterns. In addition to those likely pan-neuroblast transcription factors, there exist many transcription factors selectively enriched or repressed in certain neuroblasts. The unique combinations of transcription factors present in different neuroblasts may govern the diverse lineage-specific neuron fates

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AEC II cell differentiation in miR142 overexpressing mice

Project description:We observed that loss of miR142 leads to the increased expression of surfactant protein C and decreased Podoplanin. This indicates increased differentiation of alveolar progenitors towards AEC II lineage. In order to understand what are the genes that are being regulated during AEC II cell differentiation in miR142 overexpressing mice we are performing this experiment.

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AEC II cell differentiation in miR142 overexpressing mice

Project description:AEC II cell differentiation in miR142 overexpressing mice

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Transcription profiling of mouse postnatal neuroblasts from posterior and anterior rostral migratory streams

Project description:Postnatal neuroblasts were microdissected by laser micro dissection from posterior and anterior rostral migratory stream (RMS). Neuroblasts in the posterior RMS are just started their migration. Neuroblasts in the anterior RMS are already migrated for several days in the stream

2008-09-01 | E-MEXP-1430 | biostudies-arrayexpress

Transcription profiling of GR-N-91 neuroblasts injected subcutaneously into nude mice and a primary tumor Xenograft (PTX) was isolated whilst metastatic neuroblasts were isolated from Myocardium (MY) and Bone Marrow (BM)

Project description:We have used an IGR-N-91 parental cell line established from an involved bone marrow harvested from a high-risk NB (stage 4-NB, 8 year-old boy). These IGR-N-91 neuroblasts were injected subcutaneously into nude mice and a Primary Tumor Xenograft (PTX) was isolated whilst metastatic neuroblasts were isolated from Myocardium (MY) and Bone Marrow (BM). These malignant neuroblasts were further cultured in vitro on bovine corneal extra-cellular matrix to establish sublines (IGR/PTX, IGR/BM and IGR/MY). Agilent oligo microarray analysis was performed to compare gene expression profiles in BM and MY metastatic versus PTX neuroblasts. This analysis used eight microarrays (two replicates plus two dye-swap experiments for both MY and BM cell lines).

2006-09-11 | E-TABM-44 | biostudies-arrayexpress

Transcription profiling of human normal neuroblasts vs. malignant neuroblastomas (low- and high-stage)

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CAR-macrophages for the Treatment of HER2 Overexpressing Solid Tumors

Project description:Phase 1, first-in-human, open label study of CAR macrophages in HER2 overexpressing solid tumors.

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A comprehensive temporal patterning gene network in Drosophila medulla neuroblasts revealed by single-cell RNA sequencing

Project description:During development, neural stem cells are temporally patterned to sequentially generate a variety of neural types before exiting the cell cycle. Temporal patterning is well-studied in Drosophila, where neural stem cells called neuroblasts sequentially express cascades of Temporal Transcription Factors (TTFs) to control the birth-order dependent neural specification. However, currently known TTFs were mostly identified through candidate antibody screening and may not be complete. In addition, many fundamental questions remain concerning the TTF cascade initiation, progression, and termination. It is also not known why temporal progression only happens in neuroblasts but not in their differentiated progeny. In this work, we performed single-cell RNA sequencing of Drosophila medulla neuroblasts of all ages to study the temporal patterning process with single-cell resolution. Our scRNA-seq data revealed that sets of genes involved in different biological processes show high to low or low to high gradients as neuroblasts age. We also identified a list of novel TTFs, and experimentally characterized their roles in the temporal progression and neural fate specification. Our study revealed a comprehensive temporal gene network that patterns medulla neuroblasts from start to end. Furthermore, we found that the progression and termination of this temporal cascade also require transcription factors differentially expressed along the differentiation axis (neuroblasts -> -> neurons). Lola proteins function as a speed modulator of temporal progression in neuroblasts; while Nerfin-1, a factor required to suppress de-differentiation in post-mitotic neurons, acts at the final temporal stage together with the last TTF of the cascade, to promote the switch to gliogenesis and the cell cycle exit. Our comprehensive study of the medulla neuroblast temporal cascade illustrated mechanisms that might be conserved in the temporal patterning of neural stem cells.

2021-12-27 | GSE168553 | GEO

RNA-seq transcriptomic analysis of mouse sympathetic neuroblasts and adrenal chromaffin cells

Project description:Adrenal chromaffin cells and sympathetic neuron are derived from neural crest precursors and both cell types can give rise to childhood cancer, neuroblastoma. However only limited is known about the mechanism of their development. Better understanding of their transcriptomic profiles during development could gives an insight into the cell fates acquisition as well as the origin of neuroblastoma. Yellow fluorescent protein expressing sympathetic neuroblasts and adrenal chromaffin cells were isolated from E12.5 TH-IRES-Cre;ROSA26-EYFP mouse embryos by fluorescence-activated cell sorting. Transcriptomic profiles of sympathetic neuroblasts and adrenal chromaffin cells from embryonic age (E)12.5 TH-IRES-Cre;ROSA26-EYFP mice were generated by RNA sequencing, in four paired biological replicates.

2019-05-24 | E-MTAB-7086 | biostudies-arrayexpress

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