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MicroRNA identification in the Mycoplasma bovis infected mammary gland tissue in Holstein Friesian cattle

ABSTRACT: microRNA identification in the Mycoplasma bovis infected mammary gland tissue in Holstein Friesian cattle

PROVIDER: PRJNA640666 | ENA |

REPOSITORIES: ENA

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microRNA identification in the Mycoplasma bovis infected mammary gland tissue in Holstein Friesian cattle

Project description:The immune response associated with mastitis caused by Mycoplasma bovis is a very complicated biological process in several type of cells, including immune cells, mammary epithelial cells and, endothelial cells. Thus, revealing of the microRNAs in the Mycoplasma bovis infected mammary gland tissues is particularly important for the immune response mechanism to Mycoplasma bovis. Firstly, mammary gland tissue samples were collected from Holstein cows and screened for Mycoplasma bovis. Then, total RNA was isolated from mycoplasma bovis infected tissues and RNA sequencing was performed. After bioinformatics analysis, GO and KEGG analysis of target genes of identified microRNAs were conducted. Our results revaled that 24 of the known microRNAs were expressed differently and 13 of the novel microRNAs were expressed differently in Mycoplasma bovis positive tissues. The target genes of these microRNAs were found to be associated with especially inflammation pathways. In conclusion, this study demonstrated that identified miRNAs may be involved in the signaling pathways during mastitis case caused by Mycoplasma bovis.

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miRNA expression data from post-partum dairy cattle (Holstein Friesian) liver

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RNA-seq transcriptional profiling of peripheral blood leukocytes from cattle infected with Mycobacterium bovis

Project description:In the present study, we have analyzed the peripheral blood leukocyte (PBL) transcriptome of eight natural M. bovis-infected and eight age- and sex-matched non-infected control Holstein-Friesian animals using RNA-seq. In addition, we compared gene expression profiles generated using RNA-seq with those previously generated using the high-density AffymetrixM-BM-. GeneChipM-BM-. Bovine Genome Array platform from the same PBL-extracted RNA Identify changes occuring at the transcriptome level of PBL in response to M. bovis

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