Project description:We address the function of HDAC3 in skeletal muscle metabolism We performed HDAC3 ChIP-seq, RNA-seq, and GRO-seq in mouse muscles at different times of the day and compared between WT and HDAC3-depleted muscles.
Project description:China produces more than 77.9% of the world's production of silver carp in 2020 with the production of 3812.9 kiloton.3 The high consumption of silver carp in China is mainly by using its edible muscles for manufacture of surimi-based seafoods or other muscle foods, which may contribute high quality protein resource and other valuable nutrients in human diets. This project is to understanding the muscle composition of slaughtered fish skeletal muscle use the proteomics methods. The proteomics was performed based on the improved DDA experiment with extensive fractionation and prolonged separation of peptides, and protein searching database was informed by the Iso-seq transcriptomics.
Project description:Grouper is an important commercial maricultural fish, which suffer viral nervous necrosis (VNN) disease at the larval and juvenile stages, but the changes of transcriptomics and proteomics during viral infection remain unknown. In this study, we applied RNA-seq and label-free mass spectrum for the first time to depict the map of transcriptomics and proteomics in non-infected, susceptible-infected and tolerate-infected grouper in larval stage. Further analyses showed that the transcriptome and proteome change dramatically among 3 distinct groups, indicating that different immune response for infected and perststent grouper in larval stage. These valuable transcriptomics and proteomics datasets enable the investigation of molecular mechanism in nervous necrosis (VNN) virus infection, thus helps the further development of molecular breeding and marine fishery
