Project description:Gynandropsis gynandra seedlings were germinated then grown in the dark for seven days on 1/2 MS medium, 22oC. Tissue samples were harvested after 0 and 6 hours exposure to WL (100 mol m2 s1) and frozen at -80oC. RNA was prepared from samples using a QIAGEN Plant RNeasy extraction kit and libraries generated using TruSeq RNA Sample v2 kit. Sequencing was performed at the Department of Biochemistry, University of Cambridge using a NextSeq500 Mid Output 150 cycle.
Project description:Cleome gynandra leaf development RNA-seq RNA-seq have been performed on developing (2mm length) and mature (10mm length) leaves of the C4 photosynthesis plant Cleome gynandra. 6 runs of Illumina 36-42 bp single end have been performed ArrayExpress Release Date: 2010-12-01 Publication Author List: Sylvain Aubry, Mali Salmon, Kim M Rutherford, Paul Bertone, Andrea Brautigam, Andreas PM Weber, Krystyna A Kelly, Julian M Hibberd Publication Title: De novo transcriptome assembly enables quantitative expression analysis in non-sequenced model organisms Person Roles: submitter Person Last Name: Aubry Person First Name: Sylvain Person Mid Initials: Person Email: sa530@cam.ac.uk Person Phone: +441223 330 220 Person Address: Department of Plant Sciences. University of Cambridge. Downing Street. CB2 3EA Cambridge Person Affiliation: University of Cambridge
Project description:We isolated and compared transcriptomes of GC and M from C3 T. hassleriana and C4 G. gynandra. This was achieved using laser-capture microdissection of each cell type from fixed paradermal sections. At least 2,500 cells were isolated for each replicate. RNA was extracted, amplified and subjected to RNAseq.
Project description:RNA-seq have been performed on developing (2mm length) and mature (10mm length) leaves of the C4 photosynthesis plant Cleome gynandra. 6 runs of Illumina 36-42 bp single end have been performed
