Project description:To understand gene expression changes in different regions of the Drosophila brain with age, we performed RNAseq different regions of the adult drosophila CNS at different ages.
Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from imaginal disc / brain, female body and male body. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: small RNA discovery and profiling For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster adult bodies. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.
Project description:Study of genome-wide differential gene expression of adult Drosophila melanogaster prickle-prickle mutant brains compared to control animals.
Project description:Study of genome-wide differential gene expression of adult Drosophila melanogaster prickle-spiny legs mutant brains compared to control animals.
Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from imaginal disc / brain, female body and male body. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: small RNA discovery and profiling For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Project description:A spectral library was built for Drosophila melanogaster. The spectral library allows reproducible quantification for thousands of peptides per SWATH-MS analysis.
Proteins from Drosophila melanogaster embryo, adult flies were digested with trypsin using in-gel digestion and the peptides were fractionated by high-pH reverse phase chromatography. HRM peptides were spiked into the peptides mixture and each fraction was injected on a Sciex TripleTOF 6600 mass spectrometer fitted with microflow set-up.
The resulting .wiff files were analysed using MaxQuant and Spectronaut.
Project description:We identified genes whose expression is upregulated in adult Drosophila m. brain 48h after a stab lesion by microarray analysis of RNA extracted from injured and intact wild-type brains (Oregon-R).
Project description:dG9a-depletion decreases the starvation resistance in the adult stage of Drosophila melanogaster. To determine which genes are regulated by dG9a under starvation stress, we examined mRNA levels by performing RNA-sequence analysis using 0 h and 12 h starved dG9a null mutant and wild type as a control.