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Small RNA next-generation sequencing of cardiac fibroblasts isolated from young and old mice

ABSTRACT: Small RNA next-generation sequencing of cardiac fibroblasts isolated from young and old mice

PROVIDER: PRJNA641788 | ENA |

REPOSITORIES: ENA

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Small RNA next-generation sequencing of cardiac fibroblasts isolated from young and old mice

Project description:MiRNA expression profile was assessed in cardiac fibroblasts isolated from young (2 months old) and aged (16 months old) mice, by next generation sequencing of small RNA. Bioinformatic analysis of next-generation sequencing output demonstrated 88 differentially expressed miRNAs in aged mice - derived cardiac fibroblasts versus young mice-derived cells, of which two thirds were downregulated and one third were upregulated. The majority of downregulated miRNAs were mapped to chromosome 12, as part of Meg3-Mirg locus, while the remaining were spread on all chromosomes. Almost 90% of miRNA genes within Meg3-Mirg locus were expressed over a wide range in cardiac fibroblasts and had a decreasing trend in the aged group, the majority being significantly downregulated. This study provides evidence that aging is associated to significant alterations in miRNA expression profile in cardiac fibroblasts, a predominant feature being the downregulation of miRNAs within Meg3-Mirg locus.

2021-07-14 | GSE153214 | GEO

mRNA expression of mid-old fibroblasts co-cultured with young and mid-old fibroblasts

Project description:Examination of the difference in mRNA expression profile between mid-old fibroblasts co-cultured with mid-old fibroblasts and mid-old fibroblasts co-cultured with young fibroblasts

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mRNA expression of young, mid-old, and old fibroblasts

Project description:Examination of the difference in mRNA expression profile between young, mid-old, and old fibroblasts

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SAGE analysis of cardiac fibroblasts isolated from adult mouse heart

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SAGE analysis of cardiac fibroblasts isolated from adult mouse heart

Project description:Cardiac fibroblasts were isolated from 3-month old C57BL/6 mice. RNA was prepared from quiescent, serum-starved cardiac fibroblasts in passage 15. A total number of 118654 tags was sequenced. After removal of duplicate ditags and linker-derived sequences 110169 tags remained. This GEO Series was created by the GEO staff as part of a cleanup effort to ensure that all GEO Samples are included within a Series entry.

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Methylation profiling of directly converted human fibroblasts from young, adult and old donors into oligodendrocytes (dc-hiOL) to study age-associated changes.

Project description:This study comprises DNA methylation profiles of 9 directly converted human fibroblasts from young, adult and old donors into oligodendrocytes (dc-hiOL).

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TMT isobaric profiling of young versus old haematopoietic stem cells

Project description:Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spectra were used for peptide identification and quantitation based on TMT reporter ions. TMT isobaric comparison of old versus young haematopoietic stem and progenitor cells. Young 1 and Young 2 are samples 126 and 128 of dataset UTH_1. Old 1 and Old 2 are samples 129 and 130 of UTH_1. Young 3 is sample 131 and Old 3 is sample 130 of dataset UTH_4.

2023-10-16 | MSV000093126 | MassIVE

MicroRNA data in the hearts from young (8 week-old) and middle-aged (15 month-old) male mice

Project description:MicroRNAs have been reported to be involved in the regulation of cellular and organismal aging. However, little is known about the role of microRNAs in cardiac aging. We used microarrays to examine microRNA profiles in the hearts from young (8 week-old) and middle-aged (15 month-old) male mice.

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Transcriptome analysis of young and old zebrafish ventricles

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Comparison of expression profile between fibroblasts taken from young (23 years old) and aged (70 years old) donors

Project description:Comparison of expression profile between fibroblasts taken from young (23 years old) and aged (70 years old) donors

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