Project description:In order to identify metabolites descriptive of alterations of the working themperature during the process of anaerobic digestion, we performed untargeted metabolomics on samples of sewage sludge collected from two reactors working in parallel but with different temperature settings.
Project description:.RAW files and Compound Discoverer peak lists used for a manuscript regarding changes the chemical fingerprint of sewage sludge during the COVID-19 pandemic
2021-01-08 | MSV000086676 | MassIVE
Project description:Prokaryotic diversity on sewage sludge and anaerobic digesters
| PRJNA732219 | ENA
Project description:Sewage sludge stabilization methods alter soil microbes
Project description:.RAW files and Compound Discoverer peak lists used for a manuscript regarding changes the chemical fingerprint of sewage sludge during the COVID-19 pandemic
2021-01-08 | MSV000086676 | GNPS
Project description:municipal sewage sludge community diversity
| PRJNA847265 | ENA
Project description:Metagenomic evolution during thermal hydrolysis pretreatment anaerobic digestion of sewage sludge
Project description:Manufactured nanomaterials (MNMs) are increasingly incorporated into consumer products that are disposed into sewage. In wastewater treatment, MNMs adsorb to activated sludge biomass where they may impact biological wastewater treatment performance, including nutrient removal. Here, we studied MNM effects on bacterial polyhydroxyalkanoate (PHA), specifically polyhydroxybutyrate (PHB), biosynthesis because of its importance to enhanced biological phosphorus (P) removal (EBPR). Activated sludge was sampled from an anoxic selector of a municipal wastewater treatment plant (WWTP), and PHB-containing bacteria were concentrated by density gradient centrifugation. After starvation to decrease intracellular PHB stores, bacteria were nutritionally augmented to promote PHB biosynthesis while being exposed to either MNMs (TiO2 or Ag) or to Ag salts (each at a concentration of 5 mg L-1). Cellular PHB concentration and PhyloChip community composition were analyzed. The final bacterial community composition differed from activated sludge, demonstrating that laboratory enrichment was selective. Still, PHB was synthesized to near-activated sludge levels. Ag salts altered final bacterial communities, although MNMs did not. PHB biosynthesis was diminished with Ag (salt or MNMs), indicating the potential for Ag-MNMs to physiologically impact EBPR through the effects of dissolved Ag ions on PHB producers.