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Glutamine synthetase gene glnA plays an important role in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

ABSTRACT: Glutamine synthetase gene glnA plays an important role in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

PROVIDER: PRJNA642865 | ENA |

REPOSITORIES: ENA

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Glutamine synthetase gene glnA plays an important role in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

Project description:Purpose： The goal of this study is compare the effect of glnA gene in curdlan synthesis in Agrobacterium sp. CGMCC 11546. methods: The transcriptional and metabolomics analysis the function of glnA in Agrobacterium sp. CGMCC 11546. Results: The transcriptional and metabolomics showed that the decrease of curdlan production in the ΔglnA mutants may be caused by the insufficient supply of energy ATP conclusion: glnA play an important role in curdlan synthesis in Agrobacterium sp. CGMCC 11546

2022-06-30 | GSE153505 | GEO

phbC plays an important role in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

Project description:Purpose： The goal of this study is compare the effect of phbC gene in curdlan synthesis in Agrobacterium sp. CGMCC 11546. methods: The transcriptional and metabolomics analysis the function of phbC in Agrobacterium sp. CGMCC 11546. Results:The transcriptional and metabolomics showed that the decrease of curdlan production in the ΔphbC mutants may be caused by the insufficient supply of energy ATP conclusion:phbC play an important role in curdlan synthesis in Agrobacterium sp. CGMCC 11546

2022-06-30 | GSE153504 | GEO

Methionine biosynthetic pathway genes metH and metZ affect curdlan synthesis in Agrobacterium sp. CGMCC 11546

Project description:Purpose: The goal of this study is compare the effect of MetH and MetZ gene in curdlan synthesis in Agrobacterium sp. CGMCC 11546. methods: The transcriptional and metabolomics analysis the function of metH and metZ in Agrobacterium sp. CGMCC 11546. Results: The transcriptional and metabolomics showed that the decrease of curdlan production in the ΔmetH and ΔmetZ mutants may be caused by the insufficient supply of energy ATP conclusion: MetH and MetZ play an important role in curdlan synthesis in Agrobacterium sp. CGMCC 11546

2021-06-30 | GSE131215 | GEO

Differentially gene expression analysis after 7 hours and 24 hours fermentation of curdlan in Agrobacterium sp. CGMCC 11546

Project description:We report the analysis of differentially gene expression after 7 hours and 24 hours fermentation of curdlan in Agrobacterium sp. CGMCC 11546.

2021-06-30 | GSE132210 | GEO

phbC and glnA play important roles in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

Project description:phbC and glnA play important roles in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

| PRJNA642864 | ENA

phbC plays an important role in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

Project description:phbC plays an important role in curdlan biosynthesis of Agrobacterium sp. CGMCC 11546

| PRJNA642866 | ENA

Cytosolic glutamine synthetase 1;1 knockout effects on rice roots

Project description:Goal of the study is to characterize distinct function(s) of two cytosolic glutamine synthetase (GS) in rice plants. We grew rice lacking GS1;1 and GS1;2 under the ammonium sufficient condition. We harvested roots from the two mutants as well as those of the corresponding control.

2019-04-01 | E-MTAB-6303 | biostudies-arrayexpress

Gene expression of cytosolic glutamine synthetase 1;1 knock-out mutant rice

Project description:Rice grown in paddy fields prefers to use ammonium ions as a major source of inorganic nitrogen. Glutamine synthetase (GS) catalyzes the conversion of ammonium ions to glutamine. In three cytosolic GS in rice, OsGS1;1 has the critical role for normal growth and grain filling. To understand a role of GS1;1, we performed transcriptional profiling of wild type Nipponbare and GS1;1 mutant plants in seedling using the Agilent Rice Oligo Microarray.

2010-03-01 | GSE15122 | GEO

Methionine Sulfoximine Resistance in Mycobacterium tuberculosis Is Due to a Single Nucleotide Deletion Resulting in Increased Expression of the Major Glutamine Synthetase, GlnA1.

Project description:We investigated the effect of methionine sulfoximine (MetSox), a potent inhibitor of glutamine synthetase, on Mycobacterium tuberculosis. M. tuberculosis encodes four glutamine synthetases, of which MetSox targets the type I enzyme encoded by glnA1. Trancriptional profiling revealed that glutamate synthetase (gltB) and a type II glutamine synthetase (glnA3) were induced after exposure to MetSox. In addition, we observed a high rate (10(-5)) of spontaneous resistance to MetSox. All resistant strains had a single-nucleotide deletion in the 5' region of glnA1, and Western analysis revealed that GlnA1 expression was increased in resistant as compared with sensitive strains. These data show that M. tuberculosis can respond to the effect of MetSox inhibition either by up-regulation of GlnA3 or by GlnA1. The high frequency of resistance suggests that MetSox and other compounds specifically targeting GlnA1 are not likely to become successful anti-mycobacterial agents. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-112

2011-09-06 | E-BUGS-112 | biostudies-arrayexpress

Microarray analysis of gene expression in rice lacking cytosolic glutamine synthetase 1;2

Project description:Goal of the study is to characterize specific function(s) of the cytosolic glutamine synthetase (GS) 1;2 for apical meristem induction in rice plants. To address the issue, we conducted transcript profiling toward basal parts of the knockout mutant lacking GS1;2. We used microarrays conduct transcript profiling of gs1;2 plants. Wild-type samples were used as control.

2014-11-28 | GSE62124 | GEO

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