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Differentially expressed genes during pancreatic progenitor cell differentiation and proliferation

ABSTRACT: Differentially expressed genes during pancreatic progenitor cell differentiation and proliferation

PROVIDER: PRJNA644126 | ENA |

REPOSITORIES: ENA

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Differentially expressed genes during pancreatic progenitor cell differentiation and proliferation

Project description:A small molecule, AT7867, is a proliferation-promoting factor of human iPS cell-derived pancreatic progenitor cells; yet how it promotes cell proliferation is not known. AT7867 induces proliferation of pancreatic progenitor cells, but not that of definitive endoderm cells or primitive gut tube cells suggesting a cell type-specific effect. Our aim is to perform RNA-seq of cells obtained during a three stage differentiation of hiPS cells to the endodermal cell lineage in addition to pancreatic progenitor cells treated with AT7867, and analyze signaling pathways altered by the compound treatment.

2020-07-06 | GSE153806 | GEO

Differentially expressed genes during pancreatic bud differentiation in cellular aggregates

Project description:Analysis of pathways altered by aggregation culture during differentiation from pancreatic progenitor cells into pancreatic bud cells. The hypothesis tested in the present study was that the high-cell density culture induces specific signals to facilitate the differentiation into pancreatic bud cells. Results provide the differentially regulated pathways between the low-cell density cultures and the high-cell density cultures.

2017-06-21 | GSE83816 | GEO

Transcription profiling of mouse wild type and Ipf1/Pdx1 null mutated pancreatic progenitor cells

Project description:Identification of differentially expressed genes in Ipf1/Pdx1 null mutated mouse pancreatic progenitor cells.

2008-02-12 | E-TABM-280 | biostudies-arrayexpress

Analysis of differentially expressed genes between control and shTAK1 pancreatic cancer cells

Project description:Analysis of differentially expressed genes between control and shTAK1 pancreatic cancer cells

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A set of 72 differentially expressed miRNAs provide a molecular signature for pancreatic cancer

Project description:MicroRNAs (miRNAs) are short single-stranded RNA molecules that have a critical role in the regulation of gene expression. Alterations in miRNA expression levels have been observed in multiple tumor types and there is clear evidence on their active involvement in cancer development. Here, a comprehensive miRNA expression profiling in 16 pancreatic cancer cell lines and four normal pancreatic samples provided a specific molecular signature for pancreatic cancer and enabled us to identify 72 differentially expressed miRNAs with approximately half of them being up- and half downregulated in cancer cells as compared to normal samples. Of these, miR-31 was selected for further functional analyses based on its interesting M-bM-^@M-^\on-offM-bM-^@M-^] type expression profile, i.e. very low or even absent expression in normal pancreas and in six of the pancreatic cancer samples but extremely high expression in the remaining ten cell lines. Quite unexpectedly, both the inhibition of miR-31 in AsPC-1 and HPAF-II pancreatic cancer cells with high endogenous expression and forced expression of miR-31 in MIA PaCa-2 with low endogenous levels led to reduced cell proliferation, migration and invasion. More importantly, in AsPC-1 cells further enhancement of miR-31 also resulted in reduced cell migration and invasion, implicating that the level of miR-31 is critical for these phenotypes. We also identified novel miR-31 target genes, APBB2 and RSBN1, that might contribute to cancer pathogenesis. This study highlights a specific miRNA expression pattern in pancreatic cancer and reveals that manipulation of miR-31 expression leads to reduced cell migration and invasion in pancreatic cancer. 16 pancreatic cancer cell lines and 4 normal pancreatic RNA samples were hybridized on Agilent vs1 miRNA microarrays. Pool of four normal samples was hybridized on each slide to allow normalization between slides.

2012-03-30 | E-GEOD-28955 | biostudies-arrayexpress

Differentially expressed genes of C2C12 cells influenced by murine pancreatic cancer derived exosomes

Project description:Mice pancreatic cancer derived exosomes(KPC-exosomes) could induce skeletal muscle cells(C2C12) insulin resistance and exosomal microRNAs (miRNAs) may exert an important effect (Sci Rep. 2017;7(1):5384). The data set GSE95741 provided the differentially expressed micro RNAs. In this work, we aim to detect the differentially expressed genes and explore the intrinsic link.

2022-01-01 | GSE174058 | GEO

Differentially expressed genes in pancreatic cancer cell with erastin and vitamin C treatment

Project description:Differentially expressed genes in pancreatic cancer cell with erastin and vitamin C treatment

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Differentially expressed genes of C2C12 cells influenced by murine pancreatic cancer derived exosomes

Project description:Differentially expressed genes of C2C12 cells influenced by murine pancreatic cancer derived exosomes

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WNT7 Promotes Proliferation of Pancreatic Progenitor Cells Differentiated from Human Pluripotent Stem Cells through Non-Canonical Wnt Signaling Pathway

Project description:A small molecule, AT7867, promotes proliferation of human pluripotent stem cell-derived pancreatic progenitor cells (PPCs), yet detailed mechanisms of its proliferative effect were not fully understood. Here, we performed cell-based siRNA screening on a subset of genes to reveal that WNT7B is a growth factor of human PPCs as previously shown in mouse pancreas development. Although recombinant proteins had no in vitro activity, feeder cell lines stably expressing Wnt7a or Wnt7b enhanced PPC proliferation. Further analyses showed that canonical Wnt signaling pathway was not affected by AT7867 treatment as well as Wnt7a and Wnt7b. Knockdown of a non-canonical Wnt pathway component, PKCα, and its downstream substrate, MARCKS, blocked PPC proliferation suggesting a requirement of this pathway. Phosphoproteome analysis revealed that AT7867 inhibited Yin Yang 1 (YY1) phosphorylation at Ser118, which directly or indirectly regulated expression of WNT7B in PPCs. Taken together, non-canonical Wnt signaling mediated by WNT7B plays a critical role for the expansion of human PPC population that is a promising alternative cell source to generate β cells for the cure of diabetes.

2021-07-01 | PXD018221 | JPOST Repository

WNT7 Promotes Proliferation of Pancreatic Progenitor Cells Differentiated from Human Pluripotent Stem Cells through Non-Canonical Wnt Signaling Pathway

2022-03-27 | PXD018222 | JPOST Repository

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