Project description:Bovine respiratory epithelial cells have different susceptibility to bovine respiratory syncytial virus infection. The cells derived from the lower respiratory tract were significantly more susceptible to the virus than those derived from the upper respiratory tract. Pre-infection with virus of lower respiratory tract with increased adherence of P. multocida; this was not the case for upper tract. However, the molecular mechanisms of enhanced bacterial adherence are not completely understood. To investigate whether virus infection regulates the cellular adherence receptor on bovine trachea-, bronchus- and lung-epithelial cells, we performed proteomic analyses.

2020-07-28 | PXD019509 | JPOST Repository

Evolutionary history of Mexican domesticated and wild Meleagris gallopavo.

Project description: Not available

| S-EPMC5905111 | biostudies-literature

Meleagris gallopovo

Project description:Oviduct of turkeys

| PRJNA810919 | ENA

Influenza A H1N1 induced disturbance of the respiratory and gastrointestinal microbiome - a multi-omics characterization

Project description:Seasonal influenza outbreaks represent a large burden for the healthcare system as well as the economy. While the role of the microbiome in the context of various diseases has been elucidated, the effects on the respiratory and gastrointestinal microbiome during influenza illness is largely unknown. Therefore, this study aimed to characterize the temporal development of the respiratory and gastrointestinal microbiome of swine using a multi-omics approach prior and during influenza infection. Swine is a suitable animal model for influenza research, as it is closely related to humans and a natural host for influenza viruses. Our results showed that IAV infection resulted in significant changes in the abundance of Moraxellaceae and Pasteurellaceae families in the upper respiratory tract. To our surprise, temporal development of the respiratory microbiome was not affected. Furthermore, we observed significantly altered microbial richness and diversity in the gastrointestinal microbiome after IAV infection. In particular, we found increased abundances of Prevotellaceae, while Clostridiaceae and Lachnospiraceae decreased. Furthermore, metaproteomics showed that the functional composition of the microbiome, known to be robust and stable under healthy conditions, was heavily affected by the influenza infection. Metabolome analysis proved increased amounts of short-chain fatty acids in the gastrointestinal tract, which might be involved in faster recovery. Furthermore, metaproteome data suggest a possible immune response towards flagellated Clostridia induced during the infection. Therefore, it can be assumed that the respiratory infection with IAV caused a systemic effect in the porcine host and microbiome.

2022-02-16 | PXD024077 | Pride

Upper respiratory microbiome of COVID-19 patients

Project description:Understanding the pathology of COVID-19 is a global research priority. Early evidence suggests that the microbiome may be playing a role in disease progression, yet current studies report contradictory results. Here, we examine potential confounders in COVID-19 microbiome studies by analyzing the upper respiratory tract microbiome in well-phenotyped COVID-19 patients and controls combining microbiome sequencing, viral load determination, and immunoprofiling. We found that time in the intensive care unit and the type of oxygen support explained the most variation within the upper respiratory tract microbiome, dwarfing (non-significant) effects from viral load, disease severity, and immune status. Specifically, mechanical ventilation was linked to altered community structure, lower species- and higher strain-level diversity, and significant shifts in oral taxa previously associated with COVID-19.

| EGAS00001004951 | EGA

upper respiratory tract metagenome

Project description:Upper respiratory tract microbiota Metagenome

| PRJNA209575 | ENA