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Myeloid cells are enriched in tonsillar crypts providing insight into the host tropism of HPV

ABSTRACT: Myeloid cells are enriched in tonsillar crypts providing insight into the host tropism of HPV

PROVIDER: PRJNA648831 | ENA |

REPOSITORIES: ENA

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Myeloid cells are enriched in tonsillar crypts providing insight into the host tropism of HPV

Project description:Viruses are the second leading cause of cancer worldwide, and human papillomavirus (HPV)-associated head and neck cancers are increasing in incidence in the United States. HPV preferentially infects the crypts of the tonsils rather than the surface epithelium. The present study sought to characterize the unique microenvironment within the crypts to better understand the host tropism of HPV to a lymphoid rich organ. Laser-capture microdissection of distinct anatomic areas (crypts, surface epithelium, and germinal centers) of the tonsil coupled with transcriptional analysis and multi-parameter immunofluorescence staining was performed and demonstrated that the tonsillar crypts are enriched with myeloid populations which co-express multiple canonical and non-canonical immune checkpoints, including PD-L1, CTLA-4, HAVCR2 (TIM-3), ADORA2A, IDO1, BTLA, LGALS3, CDH1, CEACAM1, PVR, and C10orf54 (VISTA). The resident monocytes may foster a permissive microenvironment that facilitates HPV infection and persistence. Furthermore, the myeloid populations within HPV-associated tonsil cancers co-express the same immune checkpoints, providing insight into potential novel immunotherapeutic targets for HPV-associated head and neck cancers. The associated paper "Myeloid cells are enriched in tonsillar crypts providing insight into the host tropism of HPV", Austin K. Mattox, Jessica Roelands, Talia Saal, Yang Cheng, Darawan Rinchai, Wouter Hendrickx, Geoffrey D. Young, Thomas J. Diefenbach, Alan E. Berger, William H. Westra, Justin A. Bishop, William C. Faquin, Francesco M. Marincola, Mikael J. Pittet, Davide Bedognetti, and Sara I. Pai; The American Journal of Pathology, Online ahead of print DOI:https://doi.org/10.1016/j.ajpath.2021.06.012

2021-06-18 | GSE155139 | GEO

Gene expression data from DSS-treated crypts, normal colon crypts and tumor crypts

Project description:Comparative gene expression profiling between DSS-treated crypts and normal colon crypts Comparative gene expression profiling between normal colon crypts and tumor crypts

2017-01-01 | GSE56050 | GEO

lung and tonsillar mast cells

Project description:GeneChip Data of human lung mast cells and tonsillar mast cells Keywords: other

2004-10-20 | GSE1848 | GEO

Actinomyces denticolens colonisation identified in equine tonsillar crypts.

Project description: Not available

| S-EPMC5020674 | biostudies-literature

Transcription profiling of human lung and tonsillar mast cells

Project description:GeneChip Data of human lung mast cells and tonsillar mast cells

2008-06-11 | E-GEOD-1848 | biostudies-arrayexpress

microRNA expression in human tonsillar B cell populations

Project description:small RNA profiles of 6 human tonsillar B cell populatios (naive B cells, pre-germinal center B cells, centrocytes, centroblasts, memory B cells, and plasma cells) were determined by deep sequencing. These samples were compared to mouse developing lymphocytes, various hematopoietic cell lineages, and tissues.

2010-07-24 | GSE23090 | GEO

Distinct stage-specific transcriptional states of B cells in human tonsillar tissue [single-cell RNA-seq]

Project description:Defining B cell states in human tonsillar tissue using single cell transcriptomics.

2021-08-16 | GSE182221 | GEO

Transcriptomic analysis of differential expressed genes of human tonsillar epithelial cells UT-SCC-60B in response to EV71 infection

Project description:Purpose: The goals of this study are to understand the transcriptional changes of human tonsillar epithelial cells UT-SCC-60B in response to EV71 infection and determine the differential expressed genes Methods: Transcriptional profiles of human tonsillar epithelial cells UT-SCC-60B and human tonsillar epithelial cells infected with EV71 were generated by next generation sequencing, in triplicate, using Illumina X-ten/NovaSeq. The sequence reads that passed quality filters were analyzed abundance of mRNA using StringTie, the FPKM values of genes and transcripts were calculated by Ballgown of R software, alternative splicing events were determined by rMATS Results: Using an optimized data analysis workflow, we mapped more than 36 million sequence reads per sample. Total 11137 gene counts and 26860 transcript counts were identified in human tonsillar epithelial cells, 11347 gene counts and 27994 transcript counts were identified in EV71-infected human tonsillar epithelial cells. Total 96 genes and 499 transcripts were down-regulated, 105 genes and 550 transcripts were up-regulated according the criteria that fold changes are more than 1.5, p value less is than 0.05 and mean of FPKM value is more than 0.5 to defined as the differential expressed genes or differential expressed transcripts. Total 11563 novel genes and 14927 novel transcripts were identified and 3266 novel transcripts had the potential coding probabilities. Finally, total 5742 genes occurred alternative splicing events. Conclusions: Our study represents the first detailed analysis of the transcriptional changes of human tonsillar epithelial cells UT-SCC-60B in response to EV71 infection, this results will provide novel insight into the interaction between human tonsillar epithelial cells and EV71

2019-08-31 | GSE136668 | GEO

Transcriptomic analysis of human tonsillar TFH subsets

Project description:We used an IL4-capture assay followed by FACS sorting, to isolate IL4-secreting TFH cells from a human tonsil and compared their transcriptomic profiles with CXCR5hi PD1hi IL4-negative tonsillar TFH cells and IL4-producing CXCR5neg non-TFH cells (TH2 cells). Our studies validate the notion of functionally distinct TFH subsets and identify genes that are specifically expressed in and define the human IL-4 secreting TFH cell subset.

2018-04-03 | GSE111968 | GEO

microRNA expression in human tonsillar B cell populations

2010-07-23 | E-GEOD-23090 | biostudies-arrayexpress

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