Similar Datasets
Project description:Dimensions: MAGs from Siberian Permafrost
| PRJNA596250 | ENA
Project description:MDA-MB231 cells were transfected with control vector, RAI2-overexpression vector, or vector with RAI2-4A, which has a mutated interaction domain w.r.t. CtBP/CtBP2 Three replicates each of control condition, RAI2 overexpression or RAI2-4A overexpression.
2015-06-15 | E-GEOD-65489 | biostudies-arrayexpress
Project description:We sought to examine the mechanism through which phospho-mutants can contribute to the transformation of MCF10A acini. To investigate this, we examined the RNA abundance of Myc and Myc phospho-mutants (T58A, S71A/S81A, and Myc-4A) against a GFP control. Three independent biological replicates of the five samples (Myc, T58A, S71A/S81A, Myc-4A and GFP control) were isolated on day 4 and processed on Affymetrix HG-U133 Plus2 chips.
2013-09-24 | E-GEOD-51123 | biostudies-arrayexpress
Project description:This series includes 1 microarray used to detect parainfluenza 4a from an endotracheal aspirate from a patient with acute respiratory failure Keywords: viral detection
2006-05-01 | GSE4191 | GEO
Project description:MDA-MB231 cells were transfected with control vector, RAI2-overexpression vector, or vector with RAI2-4A, which has a mutated interaction domain w.r.t. CtBP/CtBP2
2015-06-15 | GSE65489 | GEO
Project description:Transcriptional profiling of Mycobacterium tuberculosis CDC1551 and 6-azasteroid resistant mutants ASR1 and ASR2 with treatment of 6-azasteroids, 4a and 6a, for 6 hours.
2020-08-01 | GSE118482 | GEO
Project description:To investigate type I interferon regulated genes in CD8+ T cells, we used microarray analyses after stimulation of primary murine T cell cultures. Negatively sorted T cells from naive C57Bl/6 mice were incubated with PBS or anti-CD3 in presence or absence of type I interferon (IFN-4a, 500U/mL). After 6h total RNA was extracted from the primary T cell cultures and microarrays were performed after RNA quality control. Among significantly regulated genes, we identified NK cell receptor ligands to be affected by exposure to type I interferon. 10^6 negatively sorted CD8+ T cells were stimulated with anti-CD3 antibody in the presence or absence of IFN-4a. Additionally, CD8+ cells were mock treated with PBS in the presence or absence of IFN-4a. After 6h, total RNA was extracted from cell suspensions and microarray analyses were performed. All experiments were carried out in triplicates.
2014-06-30 | E-GEOD-57421 | biostudies-arrayexpress
Project description:Individual differences in basal leukocyte gene expression profiles as a function of Big 5 personality dimensions
2015-01-28 | GSE60491 | GEO
Project description:B-cell receptor (BCR) signaling is essential for the development of B-cells and plays a critical role in B-cell neoplasia. Increasing evidence indicates an association between chronic hepatitis C virus (HCV) infection and B-cell lymphoma, however, the mechanisms by which HCV causes B-cell lymphoproliferative disorder are still unclear. Herein, we demonstrate the expression of HCV viral proteins in B-cells of HCV-infected patients and show that HCV up-regulates BCR signaling in human primary B-cells. HCV nonstructural protein NS3/4A interacts with CHK2 and down-regulates its activity, modulating HuR posttranscriptional regulation of a network of target mRNAs associated with B-cell lymphoproliferative disorders. Interestingly, the BCR signaling pathway was found to have the largest number of transcripts with increased association with HuR and was up-regulated by NS3/4A. Our study reveals a previously unidentified role of NS3/4A in regulation of host BCR signaling during HCV infection, contributing to a better understanding of the molecular mechanisms underlying HCV-associated B-cell lymphoproliferative disorders.
2015-10-14 | GSE71757 | GEO
Project description:Transcriptome analysis of total RNA samples from human MCF7 cell line. And the cells line were knocked down endogenous SRPK2 and overexpressed the SRPK2 WT or SRPK2 O-GlcNAcylation deficient 4A mutation
2022-03-01 | GSE147725 | GEO