Project description:<i>Austinograea</i> species are restricted to hydrothermal vents and are typically considered to be omnivorous predators in vent communities. Here we present the complete mitochondrial genome of <i>Austinograea</i> sp. which was collected from Carlsberg Ridge, the mid-ocean ridge in the northwestern Indian Ocean. The genome is 15,584 bp in length with a 68.11% AT content. It contains 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, and 22 transfer RNA genes. Phylogenetic analysis shows that the present species is closest to <i>Austinograea rodriguezensis</i>. This study contributes to further phylogenetic analysis within Eubrachyura.
Project description:The complete mitochondrial genome sequenced from the floral egg crab <i>Atergatis floridus</i> (Linnaeus, 1767) and the determination of the position of the species in the reconstructed phylogenetic tree of the infraorder Brachyura using the protein coding mitochondrial genes are presented. Results show the mitochondrial genome length of <i>A. floridus</i> is 16,435?bp with nucleotide distribution as 33.4% A, 20.3% C, 10.5% G and 35.8% T. The structure of the complete mitochondrial genome of the species is the same as with the previous xanthid record. The result of the phylogenetic analysis suggests that <i>A. floridus</i> is the closest species to other Xanthidae species in the brachyuran records. This is the first complete mitochondrial genome record from the genus <i>Atergatis</i>.
Project description:In this study, the complete mitochondrial genome of Norwegian skates imported to Korea was sequenced with a circular molecule of 17,121 bp, which consisted of 13 protein-coding genes (PCGs), 2 ribosomal RNAs, 22 transfer RNA genes, and a control region (D-loop). And among these sequences, 193 bp sequence in the D-loop of the genus <i>Raja</i> suggested the possibility of being used as a genetic marker for classification of <i>Raja</i> and <i>Dipturus</i> species. The BI phylogenetic tree by using the nucleotide sequences of 13 PCGs from 15 available mitogenomes of family Rajidae confirmed also that Norwegian skates imported to Korea form a group with <i>Raja brachyura</i> species with high branch value, and that this was a species of <i>Raja brachyura</i>. As above, these results would be expected to provide for the further understanding on the phylogenetic relationship, taxonomic classification and phylogeography of the family Rajidae.
Project description:The complete mitochondrial genome is sequenced and analyzed from a xanthid crab <i>Etisus anaglyptus,</i> which is the first complete mitochondrial genome for the genus. The mitochondrial genome length of <i>E. anaglyptus</i> is 16,435 bp and it is composed of 13 protein coding genes, two ribosomal RNA genes, and 22 tRNA genes. The structure and gene orientation of the mitochondrial genome is identical with the other brachyuran records. Furthermore, phylogenetic relationships of the infraorder Brachyura evaluated by mitochondrial protein coding genes. The phylogenetic study showed that <i>E. anaglyptus</i> is positioned in the superfamily Xanthoidea and the closest species to <i>E. anaglyptus</i> is <i>Leptodius sanguineus.</i>
Project description:In this study, we first obtained the complete mitochondrial genome of <i>Aparapoamon similium</i> (Decapoda: Brachyura). The complete mitochondrial genome is 19,236?bp in length and includes 37 typical genes (13 protein-coding genes, 22 tRNAs genes, 2 rRNAs genes, and 1 putative control region). The whole mitochondrial genome is characterized by the apparent AT bias (72.82%). BI and ML phylogenetic analysis based on 67 mitochondrial genomes of Brachyura species show a highly similar topology structure with high bootstrap supported. The results reveal the close relationship between <i>A. similium</i> and <i>Potamiscus motuoense.</i> This study would establish a solid data foundation for further diversification studies.
Project description:The complete mitochondrial genome of <i>Cancer pagurus</i> was obtained using next-generation sequencing. The circular genome was 42,736?bp in length, consisting of 13 protein-coding genes, 26 transfer RNA genes, and 2 ribosomal RNA genes. The control region was not found in mitochondrial genome. Of the 41 genes, 21 were encoded by the heavy strand, while the others were encoded by the light strand. The genome composition with A?+?T bias (74.10%). The phylogenetic analysis suggested that <i>C. pagurus</i> was closest to <i>Austinograea alayseae</i>. The newly described mitochondrial genome may provide valuable data for phylogenetic analysis for Cancridae.
Project description:We report the complete mitochondrial genome of <i>Bottapotamon lingchuanense</i> for the first time, which is found to be 17,612 base pairs in length, and contains 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA), and 1 non-coding AT-rich region known as the D-loop. In addition, the mitogenome has 17 intergenic regions ranging from 1 to 1512 bp in length. The mitochondrial genome of <i>B. lingchuanense</i> is the first mitochondrial genome under the genus <i>Bottapotamon</i>, providing DNA data for species identification, enriching the species diversity of Brachyura. The maximum-likelihood (ML) tree and Bayesian inference (BI) tree based on the 13 PCGs of mitochondrial genome of Brachyura species showed similar topologies with high confidence, and the analysis results were consistent with the current mainstream classification system. The results indicating that <i>B. lingchuanense</i> is closely related to <i>Neilupotamon sinense</i>, <i>Sinopotamon</i>, and <i>Tenuilapotamon</i>, and it is likely to be derived from them.
Project description:We sequenced the mitochondrial genomes of the spider crabs Maja crispata and Maja squinado (Majidae, Brachyura). Both genomes contain the whole set of 37 genes characteristic of Bilaterian genomes, encoded on both ?- and ?-strands. Both species exhibit the same gene order, which is unique among known animal genomes. In particular, all the genes located on the ?-strand form a single block. This gene order was analysed together with the other nine gene orders known for the Brachyura. Our study confirms that the most widespread gene order (BraGO) represents the plesiomorphic condition for Brachyura and was established at the onset of this clade. All other gene orders are the result of transformational pathways originating from BraGO. The different gene orders exhibit variable levels of genes rearrangements, which involve only tRNAs or all types of genes. Local homoplastic arrangements were identified, while complete gene orders remain unique and represent signatures that can have a diagnostic value. Brachyura appear to be a hot-spot of gene order diversity within the phylum Arthropoda. Our analysis, allowed to track, for the first time, the fully evolutionary pathways producing the Brachyuran gene orders. This goal was achieved by coupling sophisticated bioinformatic tools with phylogenetic analysis.
Project description:In this study, the authors first obtained the mitochondrial genome of Somanniathelphusa boyangensis. The results showed that the mitochondrial genome is 17,032bp in length, included 13 protein-coding genes, 2 rRNAs genes, 22 tRNAs genes and 1 putative control region, and it has the characteristics of the metazoan mitochondrial genome A+T bias. All tRNA genes display the typical clover-leaf secondary structure except tRNASer(AGN), which has lost the dihydroxyuridine arm. The GenBank database contains the mitochondrial genomes of representatives of approximately 22 families of Brachyura, comprising 56 species, including 4 species of freshwater crab. The authors established the phylogenetic relationships using the maximum likelihood and Bayesian inference methods. The phylogenetic relationship indicated that the molecular taxonomy of S. boyangensis is consistent with current morphological classification, and Parathelphusidae and Potamidae are derived within the freshwater clade or as part of it. In addition, the authors used the COX1 sequence of Somanniathelphusa in GenBank and the COX1 sequence of S. boyangensis to estimated the divergence time of this genus. The result displayed that the divergence time of Somanniathelphusa qiongshanensis is consistent with the separation of Hainan Island from mainland China in the Beibu Gulf, and the divergence time for Somanniathelphusa taiwanensis and Somanniathelphusa amoyensis is consistent with the separation of Taiwan Province from Mainland China at Fujian Province. These data indicate that geologic events influenced speciation of the genus Somanniathelphusa.
Project description:The genus <i>Rhinogobius</i> was widely distributed in East Asia. In the present study, the complete mitochondrial genome of <i>Rhinogobius</i> sp., possible a new species of freshwater goby from Anhui province of China, was sequenced for the first time. Sequence analysis showed that it is 16,511 bp in length with A + T content of 52.3%, consisting of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and a control region (CR). Phylogenetic analyses placed <i>Rhinogobius</i> sp. in a well-supported monophyletic cluster with other <i>Rhinogobius</i> fish and the phylogenetic position of <i>Rhinogobius</i> sp. was closer to <i>Rhinogobius cliffordpopei</i>.