Project description:The goal of the project was to study synthesis rates (SR) and mRNA levels (RA) genome wide in a series of aneuploid strains to check for the posible variation in SR and RA in the genes of the aneuploid chromosome withe regard to the rest of the genome. We used Genomic Run-On (GRO) experiment to mesaure SR and RA values.
Project description:Several aneuploid Candida glabrata strains, each bearing a unique karyotype, were spread on YPD plates. They all exhibited colony size vairations. Randmly 10 large colonies from each strain were sequenced.
Project description:Cryptococcus neoformans lab strain H99 was used to obtain brefeldin A adaptors. Transcriptomes of 4 adaptors, each bearing a unique aneuploid, were compared to H99.
Project description:We used C. albicans diploid reference strain SC5314 to obtain aneuploid colonies via selection by drugs. We got a collection of aneuploid strains bearing trisomy of each homolog of each chromosome.
Project description:Candida albicans lab strain SC5314 was used as parent to obtain tebuconazole adaptors. Two adaptors, TJ1503 and TJ1669 were aneuploid. Transcriptomes of these two strains were compared to SC5314.
Project description:In Cyptococcus neoformas, 4 aneuploid strains were spread on YPD plates. We saw colony size variations. We randomly sequenced some large colonies derived from each of the 4 aneuploid strains.
Project description:Candida albicans lab strain SC5314, which is a euploid diploid, was used to generate aneuploid fluconazole adaptors. Transcriptomes of aneuploids were compared to the euploid parent by growing cells in rich YPD broth.
Project description:We obtained ketoconazole tolerant mutants in Candida albicans after exposing the strain SC5314 to inhibitory concentration of ketoconazole. We did RNA-Seq to compare the transcriptomes of the aneuploid strains versus the parent euploid strain.