Project description:We created a knockout model of ART-27(UXT) specially in the Sertoli cells of the mouse testes. In order to see how loss of ART-27 effects the Sertoli cell transcriptome we isolated Sertoli cells from WT and KO animals. The WT cells were infected with a vector only construct, and the KO cells were infected with CRE producing construct.
Project description:Primary human Sertoli cells (hSerCs) were immortalized by transducing human TERT into the genome to produce human TERT-immortalized human Sertoli cells (hTERT hSerCs). Total RNA was isolated from both primary hSerCs and hTERT hSerCs and submitted for Affymetrix gene analysis. Gene expression differences of common Sertoli cell marker genes and xenobiotic transporters was preferentially assessed between each cell type.
Project description:To examine the function of PRC1 in postnatal Sertoli cells, we performed RNA-seq using PRC1 conditional KO and control mouse Sertoli cells. We also analyzed the distribution of H2AK119ub, H3K27me3 and RNF2 by ChIP-seq.
Project description:To investigate the gene expression regualted by MEK1/2-ERK1/2 signaling in Sertoli cells, we cultured primary Seroli cells and treated bFGF and PD0325901, which are activator and inhibitor of the signaling, respectively. Isolated primary Sertoli cells were incubated with 20ng bFGF, 10uM PD0325901 or vehicle for 24 hours and then gene expression changes were measured with microarray analysis
Project description:GGPPS was the key enzyme of mevalonate metabolic pathway which was used to synthesize the geranylgeranyl pyrophosphate (GGPP). When we deletion the GGPPS in the sertoli cell and the germ cell loss were found . We used microarrays to detail the global programme of gene expression after GGPPS deletion in sertoli cell and identified 1623 gene expression level change more than 2 times. Primary sertoli cells from 3 days old of control and KO mice for RNA extraction and hybridization on Affymetrix microarrays, every group primare sertoli cell were from more than 30 mice.
Project description:The aim of this experiment was to analyze the transcription profile in Sertoli cells from wild type and Fbxo38 knockout mice by RNA-seq methodology.