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SCF -licensed induction of a mucosal mast cell-like transcriptional program by TGF-b

ABSTRACT: SCF -licensed induction of a mucosal mast cell-like transcriptional program by TGF-b

PROVIDER: PRJNA650377 | ENA |

REPOSITORIES: ENA

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SCF -licensed induction of a mucosal mast cell-like transcriptional program by TGF-b

Project description:This study evaluates the effects of 72h stimulus with stem cell factor (SCF) (100 ng/mL) and TGF-b (1 ng/mL or 10 ng/mL), both alone and in combination, on the transcriptome of bone marrow-derived cultured mast cells (BMMCs) grown in IL-3 for 6-8 weeks. Through this approach, we find that SCF dramatically enhances TGF-b mediated upregulation of signature genes associated with mucosal mast cells, including transcripts encoding the proteases mMCP-1 (Mcpt1) and mMCP-2 (Mcpt2). Replicates indicate biologic duplicates. Samples were sequenced on an Illumina NextSeq 500.

2020-08-03 | GSE155571 | GEO

Notch signaling promotes differentiation of mouse mucosal mast cells

Project description:Intestinal mucosal mast cells are critically involved in the development of food-induced allergic disorders. However, factors that induce differentiation of mucosal mast cells in the intestinal mucosa are largely unknown. To identify factors involved in mucosal mast cell differentiation, we compared the gene expression profiles between mucosal mast cells isolated from the small intestine and bone marrow-derived mast cells cultured in the presence of TGF-Î² or Notch ligand. Mucosal mast cells were isolated from the small intestine of naÃ¯ve BALB/c mice by flow cytometry. Bone marrow-derived mast cells (BMMCs) were generated by culturing BALB/c bone marrow cells with murine interleukin-3 and stem cell factor for 3-4 weeks, and then cells were cultured for 6 days in the presence or absence of TGF-Î² or Delta-like 1 (Dll1), which is a Notch ligand. Total RNAs extracted from these cells were processed and hybridized to Affymetrix GeneChips.

2016-06-15 | E-GEOD-80139 | biostudies-arrayexpress

Notch signaling promotes differentiation of mouse mucosal mast cells

2016-06-15 | GSE80139 | GEO

MicroRNA profiling of mouse bone marrow-derived mast cells during mucosal differentiation and IgE-mediated activation

Project description:The aim of this study was to investigate microRNA expression pattern and its functional relevance on the commitment toward mucosal differentiation and on IgE-mediated activation of mast cells. To identify microRNA genes the expression of which change during the differentiation and activation of murine primary mast cells in vitro, the putative committed progenitors (c-kit+ cells isolated on day 6 from differentiating cultures), immature mast cells (BMMC), mucosal-type mast cells (MMC), and IgE-activated mast cells were compared by Agilent microRNA array. RNA was isolated by miRNeasy (Qiagen) from: 1) c-kit+ cells, isolated from differentiating cultures (in the presence of IL3 and SCF) derived from the bone marrow using MACS column purification, 2) immature BMMCs obtained by cultivation of bone marrow cells in the presence of IL3 and SCF for 4 weeks, 3) mucosal-type mast cells by additional differentiation of immature BMMCs for 5 days by supplementation of IL9 and TGFbeta, and 4) activated mast cells by presensitization with anti-DNP IgE followed by IgE-crosslinking by DNP-antigen challenge for 2 hours. Agilent microRNA microarray was run on these experimental groups. Four biological replicates were included in every experimental group.

2011-01-01 | E-GEOD-24321 | biostudies-arrayexpress

Gene expression profiling of IL-18 stimulated, in vitro differentiated mouse mucosal mast cells (MMC)

Project description:The aim of the present study was to clarify the role of IL-18 in mouse mast cell functions. Mouse mucosal mast cells (Balb/c) were differentiated in the presence of IL-3 (5 ng/ml), SCF (40 ng/ml), IL-9 (10 ng/ml) and human TGFbetaI (2 ng/ml) for >4 weeks. Cells were then stimulated by 100 ng/ml IL-18 for 2 hours to monitor the direct effect of IL-18. Treated samples were compared to the untreated ones in a paired experimental setting. Keywords: expression microarray, mast cell, IL-18

2008-09-26 | GSE9736 | GEO

Transcription profiling of mouse in vitro differentiated mucosal mast cells (MMC)after IL-18 stimulatiom

2009-01-21 | E-GEOD-9736 | biostudies-arrayexpress

Transcription profiling of mouse mucosal mast cell in vitro differentiation

Project description:The aim of the study was to find novel genes highly upregulated in mouse mucosal mast cells. To carry it out, differentiating progenitor cells were isolated on day4 of culturing and compared to mature mucosal mast cells obtained by day20. Experiment Overall Design: The experiment was carried out on two microarrays. Array2 is a biological and technical replicate (dye-swap) of array1. In both cases, 3-3 samples were pooled, but of different origin.

2008-06-14 | E-GEOD-6192 | biostudies-arrayexpress

Gene expression changes during murine mucosal mast cell in vitro differentiation

2007-01-31 | GSE6192 | GEO

Comparing control and TGF-beta treated cord blood-derived human mast cells

Project description:Cord blood-derived stem cells were in vitro cultured in the presence of 40 ng/ml SCF, 20 ng/ml IL6 and 2 microM lysophosphatidic acid for 5 week. Then mast cells were magnetically isolated and further cultured for 4 days in the presence or absence of 2 ng/ml TGF-betaI. Total RNA was isolated and processed according to the Agilent Low-input RNA Linear Amplification Kit and microarray hybridization protocol. Keywords: Agilent Whole Human Genome, dual channel, LogRatio values are log(10) values

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Transcription profiling of human treated cord blood-derived human mast cells control and TGF-beta treated

Project description:Cord blood-derived stem cells were in vitro cultured in the presence of 40 ng/ml SCF, 20 ng/ml IL6 and 2 microM lysophosphatidic acid for 5 week. Then mast cells were magnetically isolated and further cultured for 4 days in the presence or absence of 2 ng/ml TGF-betaI. Total RNA was isolated and processed according to the Agilent Low-input RNA Linear Amplification Kit and microarray hybridization protocol. Experiment Overall Design: 5 biological replicates from 5 donors and one technical replicate (dye-swap)

2008-06-13 | E-GEOD-4906 | biostudies-arrayexpress

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