AGO4 participates in germination during salinity
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ABSTRACT:
PROVIDER: PRJNA656768 | ENA |
REPOSITORIES: ENA
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Project description:AGO4 participates in germination during salinity
| PRJNA439011 | ENA
Project description:In this work we studied the participation of RdDM pathway in response to salinity during germination, we found that the RdDM promotes germination while AGO4 null mutant negatively regulates the germination process.
2020-08-13 | GSE156093 | GEO
Project description:In this work we studied the participation of RdDM pathway in response to salinity during germination, we found that the RdDM promotes germination whule AGO4 has an additional RdDM-independent activity that negatively regulates the germination process.
2019-09-30 | GSE112051 | GEO
Project description:Melatonin plays a potential role in multiple plant developmental processes and stress response. However, there are no reports regarding exogenous melatonin promoting rice seed germination under salinity and nor about the underlying molecular mechanisms at genome-wide. Here, we revealed that exogenous application of melatonin conferred roles in promoting rice seed germination under salinity. The putative molecular mechanisms of exogenous melatonin in promoting rice seed germination under high salinity were further investigated through metabolomic and transcriptomic analyses. The results state clearly that the phytohormone contents were reprogrammed, the activities of SOD, CAT, POD were enhanced, and the total antioxidant capacity was activated under salinity by exogenous melatonin. Additionally, melatonin-pre-treated seeds exhibited higher concentrations of glycosides than non-treated seeds under salinity. Furthermore, exogenous melatonin alleviated the accumulation of fatty acids induced by salinity. Genome-wide transcriptomic profiling identified 7160 transcripts that were differentially expressed in NaCl, MT100 and control. Pathway and GO term enrichment analysis revealed that genes involved in the response to oxidative stress, hormone metabolism, heme building, mitochondrion, tricarboxylic acid transformation were altered after melatonin pre-treatment under salinity. This study provides the first evidence of the protective roles of exogenous melatonin in increasing rice seed germination under salt stress, mainly via activation of antioxidants and modulation of metabolic homeostasis.
2020-01-20 | GSE143922 | GEO
Project description:In view of the continuous salinization of arable lands world-wide, there is an urgent need to better understand the mechanisms underlying plant responses to salt stress at different stages of their development. We investigated the role of calmodulin (CaM)-binding transcription activator 6 (CAMTA6) under salinity stress during early germination in Arabidopsis. These analyses suggest that ABA signaling is involved in CAMTA6-dependent salt-responsive gene expression, consistent with the ABA hyper-tolerance phenotype and the lack of HKT1 response to ABA and NaCl in the camta6 mutants.
2019-06-16 | GSE116069 | GEO
Project description:Seeds germination is seriously sensitive to salt stress. The mechanism in response to salt stress during seed germination is still little known. In this study, two genotypes of hulless barley lk621 and lk53 were selected to investigate the molecular mechanism of seeds salinity response during germination stage through RNA-seq and iTRAQ technologies
2020-05-27 | PXD016100 | Pride
Project description:Salinity is one of the main environmental stresses worldwide limiting soybean growth and yield. Seed imbibition and radical emergence are generally less affected by salinity in soybean. Towards unraveling the mechanisms underlying salt tolerance in soybean at germination stage, a comprehensive quantitative proteomic analysis of proteins from soybean embryonic axis during germination sensu stricto (GSS) under saline conditions was performed. Application of 100 and 200 mmol L-1 NaCl at GSS was significantly accompanied by the change in abundance (>2-fold) of 97 and 75 proteins, respectively. Most of these proteins were involved in three major functions, namely stress response and defense, protein turnover and protection and primary metabolism. Our results pave the way towards the identification of suitable biomarkers useful for improving salt tolerance in soybean.
2016-08-15 | MSV000080044 | MassIVE
Project description:Salinity is one of the main environmental stresses worldwide limiting soybean growth and yield. Seed imbibition and radical emergence are generally less affected by salinity in soybean. Towards unraveling the mechanisms underlying salt tolerance in soybean at germination stage, a comprehensive quantitative proteomic analysis of proteins from soybean embryonic axis during germination sensu stricto (GSS) under saline conditions was performed. Application of 100 and 200 mmol L-1 NaCl at GSS was significantly accompanied by the change in abundance (>2-fold) of 97 and 75 proteins, respectively. Most of these proteins were involved in three major functions, namely stress response and defense, protein turnover and protection and primary metabolism. Our results pave the way towards the identification of suitable biomarkers useful for improving salt tolerance in soybean.
2015-04-07 | PXD001943 | Pride
Project description:Bisulphite sequencing of salinity sensitive and salinity tolerant chickpea genotypes during salinity stress response using Illumina platform has been performed. At least 195 million reads in bisulphite sequencing were generated in each sample. Methylated cytosines in each sample were identified for their genomic location and sequence context.
2023-02-15 | GSE204729 | GEO
Project description:To investigate global changes in gene expression during B. emersonii germination process, we constructed cDNA microarrays containing 3,563 putative unique genes. Analyses were carried out during germination induced under various environmental conditions. Microarray data revealed that 26% of them are differentially expressed during germination in nutrient medium, in at least one of the time points investigated. Over 500 genes are up regulated during the time course of germination under these conditions, most of them being related to cell growth, including genes involved in protein biosynthesis, DNA transcription, energetic metabolism, carbohydrate and oligopeptide transport, and cell cycle control. On the other hand, several transcripts stored in the zoospores are down regulated during germination in nutrient medium, such as genes involved in signal transduction, amino acid transport and chromosome organization. We also compared germination induced in the presence of nutrients with that triggered either by adenine or potassium ions in inorganic salt solution. Several genes involved in cell growth that are induced during germination in nutrient medium do not show increased expression when B. emersonii zoospores germinate in inorganic solution, indicating that nutrients exert a positive regulatory effect on the transcription of these genes.
2009-01-05 | GSE12883 | GEO