Project description:During pregnancy, trophoblasts in the placenta are important in the success of pregnancy. We identify an adhesion molucule, JAM-C, which expresses on membrane of column cytotrophoblasts and regulates the differentiation and migration of trophoblasts. Primary cytotrophoblasts isolated from 1st trimester villi were transfected with JAM-C or control plasmids. RNA-seq analysis was sujected to identify differentially expressed genes and pathways potentially altered.
Project description:Seven patient paired primary human HLA-G+ extravillous trophoblasts (EVT) and Villous trophoblasts (VT) obtained from 1st trimester (7-9 weeks) villous tissue were obtained. RNA was isolated directly after isolation and purification using FACS sort for CD45-HLA-G+ (EVT) and CD45-HLA-G-EGFR1+ (VT) fractions. Expression profiles were compared to two samples of the choriocarcinoma cell line JEG-3 and four samples of decidual stromal cells (DSC) at passage 2 after cell culture.
Project description:Analysis of the molecular mechanisms of the interaction between E. faecalis and host placental barrier at gene expression level. The hypothesis tested in the present study was that E. faecalis influence the ranscriptomic profiling of human placental trophoblasts. Results provide important information of the response of human placental trophoblasts to E. faecalis, such as Several representative terms of the differentially expressed genes are involved in apoptosis, stress and stimulus response, placenta and embryonic development, immune response, and cell adhesion.
Project description:Analysis of gene expression in extravillous trophoblasts and column cells isolated from matrigel embedded 1st trimester placental explants cultured in 1%, 5% or 20% oxygen. The aim of this study is to identify differentially expressed genes and pathways potentially altered by varying oxygen conditions within anchoring column trophoblasts and invasive extravillous trophoblasts.
Project description:Primary term human trophoblasts were derived from placentas after a healthy pregnancy, and exposed to ionizing irradiation (vs sham) in vitro Primary human trophoblasts were irradiated 24 h after initial plating, defined as time zero. Cells were irradiated at 10 Gy using a Clinac 600C (Varian Medical Systems, Palo Alto, CA) with a 6 MV photon beam and a dose rate of 250 cGy/min. The flasks containing the cells were placed on 1.5 cm of bolus (a tissue equivalent material) since the maximum irradiation depth was 1.5 cm, which corresponded to the plated cell layer. Cells were analyzed 4, 8, and 24 h after irradiation or sham.
Project description:Primary term human trophoblasts were derived from placentas after a healthy pregnancy, and exposed to ionizing irradiation (vs sham) in vitro