Project description:Migratory birds are recently recognized as <i>Vibrio</i> disease vectors, but may be widespread transporters of <i>Vibrio</i> strains. We isolated <i>Vibrio cholerae (V. cholerae)</i> and <i>Vibrio metschnikovii (V. metschnikovii)</i> strains from migratory bird epidemic samples from 2017 to 2018 and isolated <i>V. metschnikovii</i> from migratory bird feces in 2019 from bird samples taken from the Inner Mongolia autonomous region of China. To investigate the evolution of these two <i>Vibrio</i> species, we sequenced the genomes of 40 <i>V. cholerae</i> strains and 34 <i>V. metschnikovii</i> strains isolated from the bird samples and compared these genomes with reference strain genomes. The pan-genome of all <i>V. cholerae</i> and <i>V. metschnikovii</i> genomes was large, with strains exhibiting considerable individual differences. A total of 2,130 and 1,352 core genes were identified in the <i>V. cholerae</i> and <i>V. metschnikovii</i> genomes, respectively, while dispensable genes accounted for 16,180 and 9,178 of all genes for the two strains, respectively. All <i>V. cholerae</i> strains isolated from the migratory birds that encoded T6SS and <i>hlyA</i> were non-O1/O139 serotypes without the ability to produce CTX. These strains also lacked the ability to produce the TCP fimbriae nor the extracellular matrix protein RbmA and could not metabolize trimetlylamine oxide (TMAO). Thus, these characteristics render them unlikely to be pandemic-inducing strains. However, a <i>V. metschnikovii</i> isolate encoding the complete T6SS system was isolated for the first time. These data provide new molecular insights into the diversity of <i>V</i>. <i>cholerae</i> and <i>V. metschnikovii</i> isolates recovered from migratory birds.
Project description:We report the first case of a postoperative wound infection caused by Vibrio metschnikovii on the lower right leg of a patient after saphenectomy. Compared to the healing of an uninfected site, that of the right leg was delayed, and a cure was achieved by intensified wound care. Several swabs taken from the infected site grew a gram-negative rod in pure culture that was identified as V. metschnikovii by the VITEK 2 system. The source of the infection was not detected; however, the absence of putative risk factors (exposure to water or shellfish or an episode of diarrhea), the profession of the patient (butcher), and the isolation of V. metschnikovii in a variety of farm animals (chicken, cattle, swine, and horses) suggest that infections caused by V. metschnikovii may be regarded as zoonotic.
Project description:The objective of this work was to isolate a microorganism producing alkaline protease that can be used as an ecofriendly alternative to chemicals in dehairing process of leather manufacture. Alkaline protease producing bacterium Vibrio metschnikovii NG155 was isolated from soil samples of leather industry. The protease was highly effective in dehairing of goat skin, completely eliminating the use of lime and sulfide. Histological studies of the skin after dehairing showed that the enzyme did not damage the collagen layer and brought good fiber opening. Absence of collagenase activity was confirmed by reacting pure collagen with the enzyme and analyzing it on SDS PAGE, which showed no degradation of collagen. The enzyme was stable in a wide range of pH (7-11) and temperature (10-50 °C), which makes it suitable for industrial application.
Project description:We isolated the novel strain Vibrio rotiferianus AM7 from the shell of an abalone. In this article, we report the complete genome sequence of this organism, which was obtained by combining Oxford Nanopore long-read and Illumina short-read sequencing data.
Project description:A multi-resistant strain of Vibrio parahaemolyticus was isolated from a tropical estuary in Rio de Janeiro, Brazil. Genome sequencing was conducted to establish the molecular basis of antibiotic resistance in this organism. The genetic content of this strain revealed it to be a non-virulent lineage that nevertheless possesses several antibiotic resistance determinants.