Project description:Smilax glabra Roxb, a traditional Chinese herb, has been widely used for folk medicine. Previous studies have found that it has various pharmacological activities, such as cytotoxic, anti-inflammation, anti-oxidant, hepatoprotective, and cardiovascular system protective activities. However, its roles in adipogenesis are poorly understood. We hypothesized that Smilax glabra Roxb and its main component may play important roles in regulating adipocyte diffrentiation and function. To test this hypothesis, we performed RNA-Seq on 3T3-L1 adipocytes treated with and without pure total flavonoids from Smilax glabra Roxb.
Project description:In the present study we carried out global and targeted metabolomics of differentiated IMR32 cells to study the neuroprotective mechanism of Yashtimadhu (Glycyrrhiza glabra L.) in rotenone induced cellular model of parkinsons disease. Our mass spectrometry data highlighted 2403 and 2934 aligned metabolites from the positive and negative modes respectively. Among the aligned metabolites, 756 metabolites from positive and 731 metabolites from negative polarity, were mapped to a known metabolite, and the others remained unassigned. A total of 1,102 non-redundant metabolites from the positive and negative modes were assigned.
