Project description:Purpose: this study provided a comprehensive sequence for a systemic view of the transcriptome between mango leaf and fruit, as well as fruit allergens, which will be useful for further genomic research studies and breeding of lower allergenic mango cultivars. Methods:Some allergens have previously been identified in mango (Mangifera indica Linn), including profilins, Bet v 1-like proteins and chitinase. In this paper, 66 potential allergen genes were identified and their relative expressions evaluated in mango fruit and leaf using Illumina RNA-Seq technology. Results:A total of 17.63Gb Clean Data was obtained.The number of %≥Q30 was above 94.58%.RNA-Seq generated 11,751,123 contigs that were assembled into 99,328 unigenes with 16,848 unigenes of >1000 bp. A total of 230,242 unigenes were annotated using public protein databases, with a cut-off E-value above 10−5, of which 27,295, 46,030, 24,227 and 14,023 unigenes were assigned to gene ontology terms, Nr, Swiss-Prot and clusters of orthologous groups, respectively. Allergens mainly belonged to pollen allergen, pathogenesis-related protein Bet v I family and NADPH-dependent FMN reductase.
Project description:Transcriptome analysis of the spongy tissue disorder in Mangifera indica Cv. Alphonso was carried out individually at two stages of ripening (Table green and mid ripe) in the healthy and the spongy mesocarp. Sequencing was carried out using Illumina technique. Total number of raw reads generated from each stage of tissue ranged from 5-7 Gb. Importantly, 30,582 transcripts were annotated and 1,218 enzyme commission number were assigned from oxidoreductase, transferase, hydrolase, ligase, lyase and isomerase classes. Various differential transcripts (p < 0.05) were highlighted within the healthy and the spongy tissue during ripening. These were involved in biological pathways related to metabolism of fatty acids, sugars, amino acids etc. The altered expression pattern of these transcripts indicated the change in primary as well as secondary metabolism of fruit under the spongy tissue condition. Interestingly, several stress related transcripts were differentially expressed in both the stages of the spongy mesocarp which has advocated the role of abiotic stress in the spongy tissue disorder.
