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Combined single-cell and spatial transcriptomics reveal the precise cellular states of postnatal cerebellar granule cells and connection with medulloblastoma

ABSTRACT: Combined single-cell and spatial transcriptomics reveal the precise cellular states of postnatal cerebellar granule cells and connection with medulloblastoma

PROVIDER: PRJNA658557 | ENA |

REPOSITORIES: ENA

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Combined single-cell and spatial transcriptomics reveal the precise cellular states of postnatal cerebellar granule cells and connection with medulloblastoma

Project description:The progenitor/differentiation states of cerebellum granule cells have been investigated for many years. Deregulation of granule cells development is associated with the pathogenesis of medulloblastoma (MB).Our study reveals the cellular and spatial organization of cerebellar granule cells and provide direct evidence showing the similarity and discrepancy between normal development and tumorigenesis.

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Project description:We performed gene-expression analysis of mouse cerebellar granule cell layer as compared to that of Purkinje cells. DNA microarray analysis detected genes in cerebellar granule cell layer, most of which are classified into functional molecule categories. Our comparative analysis between Purkinje cells and the granule cell layer showed that the characteristic expression pattern in Purkinje cells was particularly represented by M-bM-^@M-^\the neural communication systemM-bM-^@M-^] components. Pukinje cells and granule cell layer of the mouse cerebellum were collected by laser microdissection for RNA extraction and hybridization on Affymetrix microarrays.

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Gene expression of cerebellar granule cell layer

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The Transcriptional Regulator SnoN Promotes the Proliferation of Cerebellar Granule Neuron Precursors in the Postnatal Mouse Brain

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Notch signaling between cerebellar granule cell progenitors

Project description:During cerebellar development, maternal granule cell progenitors (GCPs) divide to produce not only postmitotic granule cells (GCs) but also sister GCPs. However, molecular machinery to proportionally produce distinct sister cell types from seemingly uniform GCPs is still elusive. Here we report Notch signaling makes a difference among GCPs, leading to their proportional differentiation.

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Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [scRNA-seq]

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Project description:To uncover candidates which are WDR4 downstream effectors regulating cerebellar granule neuron proliferation phenotype, we utilized quantitative proteomics analysis (TMT labeling) with granule neuron progenitors from P7 wdr4 conditional knockout (using Atoh1-Cre) and control cerebella.

2023-03-11 | PXD022319 | Pride

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