Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Adaptation of Cupriavidus metallidurans CH34 to toxic zinc concentrations involves an uncharacterized ABC-type transporter

ABSTRACT: Adaptation of Cupriavidus metallidurans CH34 to toxic zinc concentrations involves an uncharacterized ABC-type transporter

PROVIDER: PRJNA659286 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Adaptation of Cupriavidus metallidurans CH34 to toxic zinc concentrations involves an uncharacterized ABC-type transporter

Project description:Cupriavidus metallidurans CH34 is a well-studied metal-resistant β-proteobacterium and contains a battery of genes involved in the resistance and processing of numerous metals. Here, we performed a directed evolution experiment to obtain derivatives adapted to grow in the presence of zinc at concentrations above the minimal inhibitory concentration for the parental strain. Genetic characterization of one derivative, CH34ZnR, which was also more resistant to cadmium, revealed seven mutations, including inactivation of glpR coding for a DeoR-type transcriptional repressor by insertion of IS1088. The latter resulted in the constitutive expression of the neighboring ABC-type transporter. GlpR and the adjacent ABC transporter are highly similar to the glycerol operon regulator and ATP-driven glycerol importer of Rhizobium leguminosarum bv. viciae VF39, respectively. Deletion of glpR or the ABC transporter and complementation of CH34ZnR with the parental glpR gene further demonstrated that loss of GlpR function, with concomitant induction of the adjacent ABC transporter, is pivotal in the observed phenotype. Moreover, addition of glycerol, presumably by glycerol-mediated reduced GlpR activity, also promoted increased zinc and cadmium resistance. Thus, the ABC-type transporter is proposed to be a new adaptation route during exposure to toxic zinc concentrations.

2021-03-03 | GSE156826 | GEO

Adaptation of <i>Cupriavidus metallidurans</i> CH34 to Toxic Zinc Concentrations Involves an Uncharacterized ABC-Type Transporter.

Project description: Not available

| S-EPMC7912956 | biostudies-literature

Metal resistant bacterium Cupriavidus metallidurans CH34

2014-09-13 | GSE24094 | GEO

Response of Cupriavidus metallidurans CH34 to different Pt-species

Project description:Response of Cupriavidus metallidurans CH34 to cisplatin, Pt(IV)chloride and Au-NP

2016-02-27 | GSE78723 | GEO

Response of Cupriavidus metallidurans CH34 to different Au-species

Project description:Response of Cupriavidus metallidurans CH34 to NaAuCl4, Au(I)-thiomalate, Au(I)-thiosulfate, KAuCN, (KCN as control)

2013-03-15 | GSE42199 | GEO

Metal resistant bacterium Cupriavidus metallidurans CH34

Project description:Metal resistant bacterium Cupriavidus metallidurans CH34 uses non-conventional proteins to combat oxidative stress Keywords: Cupriavidus metallidurans; hydrogen peroxide; oxidative stress; Two-condition experiments. Comparing samples after challenging with oxidative stress versus non-induced samples. Biological triplicate. Each array contains 3 technical replicates.

2014-09-13 | E-GEOD-24094 | biostudies-arrayexpress

Proteomic Response of Cupriavidus metallidurans CH34 to gold stress

Project description:Proteomic response of Cupriavidus metallidurans CH34 to gold stress as in comparison to copper stress and control. Please note that 25Au in gel file names is a typo and should read 50Au.

2016-10-26 | PXD005034 | Pride

Response of Cupriavidus metallidurans CH34 to different Pt-species

Project description:Response of Cupriavidus metallidurans CH34 to cisplatin, Pt(IV)chloride and Au-NP In this study 7 different treatments were performed (first 2 as 3 replicates) to acquire expression profiles of the total genome of Cupriavidus metallidurans

2016-02-27 | E-GEOD-78723 | biostudies-arrayexpress

Response of Cupriavidus metallidurans CH34 to different Au-species

Project description:Response of Cupriavidus metallidurans CH34 to NaAuCl4, Au(I)-thiomalate, Au(I)-thiosulfate, KAuCN, (KCN as control) In this study 10 different treatments were performed (first 7 as 3 replicates) to acquire expression profiles of the total genome of Cupriavidus metallidurans

2013-03-15 | E-GEOD-42199 | biostudies-arrayexpress

Expression analysis of Cupriavidus metallidurans CH34 in presence of heavy metals

Project description:Cupriavidus metallidurans CH34 is a metal resistant beta-proteobacterium. The genome of this bacterium contain many genes involved in heavy metal resistance. Gene expression of C. metallidurans was studied after the addition of of Zn(II), Cd(II), Cu(II), Ni(II), Pb(II), Hg(II) or Co(II). Keywords: Heavy metal stress response

2007-03-16 | GSE7272 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data