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Data sets of microglia isolated from C57BL/6 WT mice and animals harboring microglial IL10Ra and TNF mutations, at steady state or after peripheral LPS challenge (RNAseq) and WT microglia with and without peripheral LPS challenge (ATACseq)

ABSTRACT: Data sets of microglia isolated from C57BL/6 WT mice and animals harboring microglial IL10Ra and TNF mutations, at steady state or after peripheral LPS challenge (RNAseq) and WT microglia with and without peripheral LPS challenge (ATACseq)

PROVIDER: PRJNA660755 | ENA |

REPOSITORIES: ENA

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Data sets of microglia isolated from C57BL/6 WT mice and animals harboring microglial IL10Ra and TNF mutations, at steady state or after peripheral LPS challenge (RNAseq) and WT microglia with and without peripheral LPS challenge (ATACseq)

Project description:To define the microglia response to septicemia, C57BL/6 WT mice and animals harboring microglial IL10Ra and TNF mutations were challenged with LPS and microglia were sorted for RNAseq analysis (WT, mutants) aor ATACseq (WT).

2020-09-02 | GSE157234 | GEO

IL-10 prevents pathological microglia hyperactivation following peripheral endotoxin challenge

Project description:Microglia, the resident macrophages of the brain parenchyma, are central players in CNS development, homeostasis and disorders. Distinct brain pathologies seem associated with discrete microglia activation modules. How microglia regain their ground state following challenges remains much less understood. Here, we explored the role of the IL-10 axis in restoring murine microglia homeostasis following peripheral endotoxin challenge. Specifically, we show that lipopolysaccharide (LPS)-challenged mice harboring IL-10 receptor-deficient microglia display neuronal impairment and succumb to fatal sickness. Addition of a microglial TNF deficiency rescues these animals, suggesting a microglia-based circuit driving pathology. Single cell transcriptome analysis revealed various IL-10 producing resident and recruited immune cell in the CNS, including most prominently Ly49D+ NK cells and neutrophils, but not microglia. Collectively, we define the kinetics of the microglia response to peripheral endotoxin challenge, including their activation and robust silencing, and highlight the critical role of non-microglial IL-10 in preventing deleterious microglia hyperactivation.

2020-10-22 | GSE157480 | GEO

IL-10 prevents pathological microglia hyperactivation following peripheral endotoxin challenge

Project description:IL-10 prevents pathological microglia hyperactivation following peripheral endotoxin challenge

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transcriptional responses of LPS-stimulated microglia in Alzheimer's disease

Project description:Neuroimmunity is present as an important mechanism in the brains of Alzheimer's patients, and microglia are a key component of the brain's innate immune response during the development and progression of Alzheimer's disease. The interferon pathway plays an important role in the pathophysiologic mechanisms of AD. RNA-seq was performed on LPS-treated microglia(LPS) and untreated microglia(WT) to further explore.

2024-03-11 | GSE261007 | GEO

Proteomic analysis of alcohol and LPS-treated primary mouse microglia

Project description:Microglia, the resident immune cells of the brain, can exhibit a broad range of activation phenotypes, many of which have been implicated in several diseases and disorders of the central nervous system including alcohol use disorders and disorders. By utilizing a method optimized for sensitive and rapid quantitative proteomic analysis of microglia involving suspension trapping (S-Trap), we were able to produce efficient and reproducible protein extraction from low cell yielding primary mouse brains. Using a ~2 h gradient on a 75 cm UPLC column with a modified data dependent acquisition method on a hybrid quadrupole-Orbitrap mass spectrometer (QE Plus), 5,062 total proteins were identified where 4,928 of those proteins were quantifiable by label-free quantitation (with 5 biological replicates). This analysis resulted in the most comprehensive proteomic dataset for ethanol- and LPS-treated primary mouse microglia to date and even expanded upon the well-characterized macrophage/microglia response to LPS treatment. This study also highlights the subtle, yet significant changes ethanol exposure can induce when compared to control. Interestingly, these changes are not consistent with the robust classical activation induced by LPS treatment, but instead align with the emerging theory that ethanol-treated microglia yield an alternative activation response. The contrast to LPS-treated microglia leads us to conclude that ethanol does not elicit a strong inflammatory response but rather might have a general inhibitory effect on multiple pathways such as phagocytosis and cell migration.

2020-04-07 | PXD014466 | Pride

RNA-seq of LPS-activated mouse microglia

Project description:Primary microglia were derived from neonatal mice and were activated via LPS+ATP treatment. test mice were treated with the drug Ladostigil and transcriptome was compared to untreated controls

2022-05-22 | E-MTAB-10450 | biostudies-arrayexpress

MeRIP-seq for microglia treated with PBS or LPS

Project description:The experiment is to demonstrate the global m6A methylation signatures and their correlation with mRNA expression patterns in microglia stimulated by LPS. The results also identified m6A modifiers with significantly altered expression, which reveals the key one that regulate the m6A modifications in microglia.

2022-12-31 | E-MTAB-11568 | biostudies-arrayexpress

RNA-Seq of mouse microglia under LPS stimulation and miR-106 overexpression

Project description:We investigated the expression patterns of transcripts in mouse primary microglia that are stimulated by LPS and transfected with miR-106b mimics. Three experiment groups were utilized in the RNA-seq, that are PBS+mimics control group, LPS+mimics group, and LPS+miR-106b mimics group. Samples were collected 2 days after LPS treatment and mimics transfection.

2021-10-31 | E-MTAB-10946 | biostudies-arrayexpress

Proteomic alterations in microglia with ischemic stroke and effect of LPS treatment

Project description:Ischemic stroke is a common acute CNS disorder leading to nearly half a million deaths per year in Europe. The high mortality is primarily owed to the limited treatment options of restoring blood flow in a narrow time window of several hours. Furthermore, inflammatory processes in the days and weeks after ischemic stroke contribute to tissue loss and neurological deficits. The key cells that influence and control this inflammatory cascade are microglia, the innate immune cells of the CNS. Microglia can be influenced and activated by e.g. lipopolysaccharide (LPS),a bacterial cell membrane component. It has been previously shown, that repetitive LPS stimuli prior to infarction (termed immunological preconditioning) lead to reduced infarct volumina in mouse models of ischemic stroke. Furthermore, our laboratory has shown, that phosphoinositide-3 kinase gamma mediates microglial functions after LPS-preconditioning. Hence, the aim of this work was to characterize proteomic alterations in microglia with (I) ischemic stroke in general in the tMCAO (transient middle cerebral artery occlusion) mouse model of ischemic stroke, (II) the influence of LPS-preconditioning on microglial proteomic alterations after tMCAO and (III) the role of PI3Ky in the microglial proteomic changes after tMCAO and preconditioning. This was done by a single LPS injection 3 days before tMCAO in wildtype mice and mice with PI3Ky knockout or knockin of the kinase dead form of PI3Ky.

2024-03-22 | PXD031930 | Pride

Microglia treatment with C6 glioma-conditioned medium or with LPS

Project description:Several independent microglia preparations performed on different days were plated onto wells of 24-well plates, and either left untreated (control), treated with the C6 glioma-conditioned medium, or treated with LPS.<br>For each culture condition, a number of RNA samples were prepared, with each sample then separately labeled and hybridized. These RNA samples were prepared from non-overlapping sets of wells from at least two microglia preparations, so they constitute biological replicates.

2012-12-14 | E-MEXP-2466 | biostudies-arrayexpress

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