Project description:The key enzymes of the kavalactone biosynthetic pathway from Piper methysticum were heterologuously expressed using agrobacterium infiltration in Nicotiana benthamiana. In addition, an isotopically labeled substrate, cinnamic acid-d6, was injected into the Nicotiana plants. Related to this study:
Pluskal, T., Torrens-Spence, M.P., Fallon, T.R. et al. The biosynthetic origin of psychoactive kavalactones in kava. Nat. Plants 5, 867 (2019). https://doi.org/10.1038/s41477-019-0474-0
Project description:In order to find the miRNAs, target genes and signaling pathways related to the dwarf phenotype induced by NlCSP1, 35S-NlCSP1 and 35S-GFP control were infiltrated into Nicotiana benthamiana. We analyzed the differentially expressed miRNAs in the infiltrated leaves (NlCSP1_Z/GFP_Z) and the upper uninfiltrated leaves (NlCSP1_O/GFP_O) of N. benthamiana. A total of 106 and 97 differentially expressed miRNAs were identified in NlCSP1_Z/GFP_Z and NlCSP1_O/GFP_O, respectively. KEGG annotation revealed a large number of differentially expressed miRNAs involved in plant hormone signal transduction, indicating that NlCSP1 changed the hormone signal pathway in N. benthamiana.
Project description:Small RNA expression from Nicotiana benthamiana leaves was profiled with the primary goal of ascertaining microRNA isoform diversity for known, conserved families. A secondary goal was to provide a baseline small RNA expression atlas for this species and tissue.
Project description:Identification of mRNAs which move over long-distances through the RNA-Seq analysis on Arabidopsis/Nicotiana benthamiana heterografts.
