Project description:To study function of Embigin in mouse embryos, five E17.5 WT and Emb-/- placentas and lungs were dissected, and the RNA was isolated and submitted to high-throughput sequencing to find differentially expressed genes between WT and Emb knock-out mouse lungs or placentas
Project description:We purified the differentiating ciliated cells from E17.5 mouse lungs and used Affymetrix microarrays to obtain their gene expression profiles.
Project description:Here, we present the fetal mouse intestine data from the project \\"Comparison of human and mouse mesenchyme identifies common and unique aspects of intestinal patterning\\". Whole intestines were harvested from fetuses from timed pregnant matings for wildtype C57BL/6 mice (Jax strain #000664). Fetal stages were confirmed according to the Theiler staging chart (https://www.emouseatlas.org/emap/ema/staging_criteria/staging_criteria.html). Whole intestines (from the common bile duct through the cecum) were collected at key stages of development (E13.5, E14.5, E15.5, E16 and E17.5). Male and female intestines from each stage were pooled and dissociated to single cells for single cell RNA sequencing as previously described (Miller et al. 2020 Dev Cell). Specifically, E13.5 was 6 intestines, E14.5 was 5 intestines, E15.5 was 4 intestines, E16 was 3 intestines and E17.5 was 3 intestines.
Project description:After quality control, sample integration, and checking the celltype specific genes, we identified major cell types as in scRNA-seq analysis. We also identified mesenchymal cell cluster by checking markers genes identified in scRNA-seq analysis.