Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide binding of Ikaros in naive CD4+ T cells and Th0 at day1

ABSTRACT: Genome-wide binding of Ikaros in naive CD4+ T cells and Th0 at day1

PROVIDER: PRJNA662892 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide binding of Ikaros in naive CD4+ T cells and Th0 at day1

Project description:The aim of the project is to determine the binding sites of Ikaros in CD4+ T cells. To perform this, CD4 naive T cells (CD4 TN; d0) either rested or activated with anti-CD3+anti-CD28 Abs for 1 day (Th0 d1) were subjected to a ChIP using anti-Ikaros Ab followed by high throughput sequencing.

2021-04-26 | GSE157811 | GEO

RNA-SEQ analysis of CD4+ T cells in presence or absence of Ikaros.

Project description:We found by microarray analysis that Ikaros is critical to limit the expression of several pro-inflammatory genes during Th17 cell polarization. To unravel the role of Ikaros in the expression of pro-inflammatory genes without Th17 polarizing cytokines, we performed an RNA-seq analysis in naive CD4+ T cells and CD4+ T cells activated with anti-CD3+anti-CD28 antibodies (Th0 condition), from WT (Ikaros f/f CD4-Cre-) and Ikaros TKO mice (Ikaros f/f CD4-Cre+).

2021-04-26 | GSE157810 | GEO

Genome wide chromatin accessibility (ATAC-SEQ) in the presence or absence of Ikaros in CD4+ T cells.

Project description:We found that Ikaros is critical to limit the expression of pro-inflammatory cytokines in TCR/CD28-activated CD4+ T cells. To understand if Ikaros could modify the chromatin accessibility in regions controling the expression of these pro-inflammatory genes, we examined the chromatin state in naive CD4+ T cells and CD4+ T cells activated with anti-CD3+anti-CD28 antibodies for 1 and 2 days, from WT (Ikaros f/f CD4-Cre-) and Ikaros TKO mice (Ikaros f/f CD4-Cre+), using the ATAC-seq approach.

2021-04-26 | GSE157812 | GEO

intact Hi-C from naive thymus-derived CD4-positive, alpha-beta T cell (ENCSR215PTV)

Project description:naive CD4+ T cell Th0 effector resting intact mnase For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2023-07-24 | GSE237369 | GEO

RNA sequencing of primary Naive CD4+ T cells and Activated Naive CD4+ T cells

Project description:RNA sequencing of the chromatin associated RNA and nucleoplasm associated RNA of Naive CD4+ T cells to identify novel chromatin associated RNAs containing TEs. RNA sequencing of Naive CD4+ T cells or Activated Naive CD4+ T cells treated with Scr or LINE1 antisense oligonucleotides (ASO).

2021-10-20 | E-MTAB-9574 | biostudies-arrayexpress

Role of Ikaros in the transcriptional signature of Th17 cells

Project description:We found that CD4+ T cells require Ikaros to promote IL-17 production when cultured in Th17 conditions. To understand why Ikaros null T cells do not produce IL-17, we analyzed the transcriptome of WT vs. Ikaros null naive CD4+ T cells both resting (day 0) or activated with anti-CD3 plus anti-CD28 antibodies, IL-6, TGFb1 and neutralizing anti-IFNg and anti-IL-4 antibodies (Th17 condition) for 1 or 2 days. Samples from 3 independent experiments were analyzed.

2021-04-26 | GSE133878 | GEO

BLUEPRINT EpiVar RNA-seq for Naive naive CD4+ T-cells

Project description:BLUEPRINT EpiVar RNA-seq for Naive naive CD4+ T-cells

| EGAS00001000752 | EGA

ChIP sequencing on human, circulating, ex vivo isolated Naive CD4+ T cells and Naive Activated CD4+ T cells

Project description:H3K36me3 ChIP sequencing performed on circulating ex vivo isolated CD4+ Naive T cells and Activated CD4+ Naive T cells

2021-10-20 | E-MTAB-9572 | biostudies-arrayexpress

intact Hi-C from activated CD4-positive, alpha-beta T cell (ENCSR923PPH)

Project description:naive CD4+ T cell Th0 effector _activated intact mnase For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2023-07-24 | GSE238027 | GEO

RNA sequencing of primary Naive CD4+ T cells and Activated Naive CD4+ T cells

Project description:RNA sequencing of primary Naive CD4+ T cells and Activated Naive CD4+ T cells

| PRJEB41930 | ENA