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Characterisation of DNA methylomic signatures of induced pluripotent stem cells during neuronal differentiation

ABSTRACT: Characterisation of DNA methylomic signatures of induced pluripotent stem cells during neuronal differentiation

PROVIDER: PRJNA663913 | ENA |

REPOSITORIES: ENA

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Characterisation of DNA methylomic signatures of induced pluripotent stem cells during neuronal differentiation

Project description:In development, inducing pluripotency and differentiation into different cell types results in global epigenetic changes, although the extent this occurs in induced pluripotent stem cell (iPSC)-based neuronal models has not been extensively characterized. We have differentiated iPSCs (33Qn1 cell line) into cortical neurons and assessed their DNA methylation profile at different time points during differentiation and maturation to identify DNA methylomic trajectories of neuronal aging. Samples were collected at four different time points, these were at the iPSC, neuronal precursor (NPC) and two different terminally differentiated neuronal time points (day 37 and day 58). We have identified loci undergoing significant DNA methylation changes throughout differentiation, have created an epigenetic trajectory signature associated with differentiation/maturation and identified highly connected and influential loci using gene-gene interaction analysis.

2021-06-03 | GSE158089 | GEO

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Project description: Not available

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Integrated methylomic and transcriptomic characterisation of postoperative systemic inflammatory dysregulation

Project description:Integrated methylomic and transcriptomic characterisation of postoperative systemic inflammatory dysregulation

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Integrated methylomic and transcriptomic characterisation of postoperative systemic inflammatory dysregulation (METHYLATION)

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Integrated methylomic and transcriptomic characterisation of postoperative systemic inflammatory dysregulation (RNA EXPRESSION)

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Exon-activated functional rescue secures neuronal transcript signatures

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Exon-activated functional rescue secures neuronal transcript signatures

Project description:The production of alternative RNA variants contributes to the tissue-specific regulation of gene expression. In the animal nervous system, a shift towards more distal 3’ processing sites generates hundreds of substantially extended messenger RNAs. These neuronal transcript signatures are crucial for nervous system development and function. However, no clear understanding of the mechanism of systematic 3’ extension in neurons has yet been achieved. Here, we report that the highly conserved RNA-binding protein ELAV globally regulates all events of neuronal 3’ end processing by directly binding to the proximal 3’ end of its target RNAs. We uncover an endogenous strategy of functional gene rescue, by which neuronal RNA signatures are safeguarded in a loss-of-function context. When not directly repressed by ELAV, the transcript encoding the ELAV paralogue FNE acquires a mini‑exon, which equips the new FNE protein with the ability to translocate to the nucleus and rescue neuronal 3’ end processing. We propose that exon-activated functional rescue is a widespread mechanism that ensures robustness of processes that are regulated by a hierarchy, rather than by redundancy, of effectors.

2020-10-01 | GSE146986 | GEO

Transcriptomic Profiling Reveals Discrete Poststroke Dementia Neuronal and Gliovascular Signatures

Project description:Transcriptomic Profiling Reveals Discrete Poststroke Dementia Neuronal and Gliovascular Signatures

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Methylomic trajectories across human

Project description:Dynamic changes to the epigenome mediate key neurobiological and cognitive processes in the central nervous system, and also play a role in transcriptional regulation during brain development. Although the importance of DNA methylation in brain development is highlighted by the neurodevelopmental deficits associated with mutations in genes including methyl-CpG binding protein 2 (MECP2), our knowledge about the specific methylomic trajectories associated with human neurodevelopment is extremely limited. Here we report an analysis of genome-wide patterns of DNA methylation in 179 human fetal cortex samples. Bisulphite converted DNA from 179 human brain samples was hybridized to the Illumina 450K Human Methylation Beadchip.

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Methylomic Profiling of BDE-47 Effects on Human Placental Cytotrophoblasts

Project description:CpG methylomic characterization of BDE-47 exposed cultured primary human cytrophoblasts (2nd trimester) undergoing differentiation in vitro.

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