Project description:Human bone marrow mesenchymal stem cells (MSCs) were co-cultured for 7 days with endothelial cells, where they participated in the formation of microcapillaries. MSCs that were exposed to the microcapillaries or kept as monocultures were isolated by FACS and analyzed by RNAseq.
Project description:The phenotype of bone marrow mesenchymal stem cells in the murine Townes model of sickle cell disease is not well characterized. We analyzed differences in the gene signature between SA and SS MSCs. MSCs were defined by CD45-Ter119-CD31-CD51+CD140a+ surface expression. We identified significant differences in SS MSC gene profile and stem cell functionality compared to SA control MSCs.
Project description:2D IDA protein quantitation of mesenchymal stem cells derived from bone
marrow across five donors. A total of 10 2D LC-MS runs were performed, using cells both not stimulated and following a 20 hour treatment with interferon gamma.
Project description:Human bone-marrow-derived mesenchymal stem cells from young and old donors and tenogenic, chondrogenic and osteogenic constructs derived from these were subject to RNASeq and miRNASeq. We wished to identify common pathways of musculoskeletal ageing.
Project description:Search for differentially expressed genes during osteoblast differentiation (0, 24h, 48h and 168h) of human bone marrow or umbilical cord mesenchymal stem cells
Project description:We cultured bone marrow haematopoietic stem and progenitor cells with bone marrow mesenchymal stromal cells to understand the interaction between the two cell types.
Project description:To identify proteins involved in the regulation of adipogenic differentiation under hypoxic conditions, an RT2 Profiler PCR array was used to screen a panel of 84 genes associated with human adipogenesis in Bone-marrow Mesenchymal stem cells under normal and hypoxic conditions. Bone-marrow Mesenchymal stem cells were put in hypoxic chamber, set at an oxygen concentration of 0.2%. Cells under normoxia are considered as a control.