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Modeling Preeclampsia using human induced pluripotent stem cells [RNA-seq]

ABSTRACT: Modeling Preeclampsia using human induced pluripotent stem cells [RNA-seq]

PROVIDER: PRJNA668942 | ENA |

REPOSITORIES: ENA

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Modeling Preeclampsia using human induced pluripotent stem cells

Project description:Modeling Preeclampsia using human induced pluripotent stem cells

| PRJNA669030 | ENA

Modeling Preeclampsia using human induced pluripotent stem cells [methylation]

Project description:Modeling Preeclampsia using human induced pluripotent stem cells [methylation]

| PRJNA669029 | ENA

Modeling Preeclampsia using human induced pluripotent stem cells [methylation]

Project description:Preeclampsia (PE) is a hypertensive disorder, which affects up to 10% of pregnancies worldwide. The primary etiology is considered to be abnormal development and function of placental cells called trophoblasts. We previously developed a two-step protocol for differentiation of human pluripotent stem cells, first into cytotrophoblast (CTB) progenitor-like cells, and then into both syncytiotrophoblast (STB)- and extravillous trophoblast (EVT)-like cells, and showed that this protocol can be used to model both normal and abnormal trophoblast differentiation. We have now applied this protocol to differentiate induced pluripotent stem cells (iPSC) derived from placentas of pregnancies with or without PE. While we did not note any differences in CTB induction, PE-iPSC-derived CTB showed a defect in syncytialization, and had a >180-fold reduction in expression of PSG4, a mature STB marker (p<0.02). While EVT formation did not appear to be altered, the invasive capacity of PE-iPSC-derived EVT was non-responsive to a decrease in oxygen tension, while that of non-PE-iPSC-derived EVT was enhanced 3.8-fold. RNA sequencing analysis showed gene expression changes consistent with defects in STB formation and response to hypoxia in PE-iPSC derived trophoblast. However, differences in DNA methylation were minimal, with only documented differences consistent with part of the response to hypoxia. Overall, PE-associated iPSC recapitulated multiple defects associated with placental dysfunction, with their lack of response to decreased oxygen tension emphasizing the importance of the interface with the maternal microenvironment in normal placentation.

2021-03-15 | GSE159479 | GEO

Modeling Preeclampsia using human induced pluripotent stem cells [RNA-seq]

2021-03-15 | GSE159437 | GEO

Modeling Preeclampsia using human induced pluripotent stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-03-15 | GSE159480 | GEO

Modeling Alcohol-induced Neurotoxicity using Human Induced Pluripotent Stem Cell-derived Three-Dimensional Cerebral Organoids

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In vitro modeling of human germ cell development using pluripotent stem cells

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Modeling sex differences in humans using isogenic induced pluripotent stem cells

Project description:We developed a cellular model to study sex differences in humans. Using somatic cells from a mosaic Kleinefelter patient, we generated isogenic human induced pluripotent stem cell lines with different sex chromosome complements – 47,XXY / 46,XX / 46,XY and 45,X0.

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Modeling Keratoconus Using Induced Pluripotent Stem Cells.

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Chorionic villus sampling (CVS) microarray in preeclampsia

Project description:4 chorionic villus sampling specimens in pregnancies destined for preeclampsia and 8 matched controls were analyzed 36 genes were differentially expressed using J5 score and prediction modeling in caGEDA computer program Keywords: snap frozen, banked CVS specimens with known pregnancy outcomes revealed genes differentially expressed in preeclampsia pregnancies

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