Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Francisella tularensis subsp. holarctica LVS strain:Live Vaccine Strain

ABSTRACT: Francisella tularensis subsp. holarctica LVS strain:Live Vaccine Strain Raw sequence reads

PROVIDER: PRJNA669398 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Francisella tularensis subsp. holarctica LVS

Project description:Francisella tularensis subsp. holarctica LVS Genome sequencing

| PRJNA390943 | ENA

Francisella tularensis subsp. holarctica LVS

Project description:Francisella tularensis subsp. holarctica LVS Genome sequencing

| PRJNA236485 | ENA

Gene expression data from F. tularensis-exposed neutrophils

Project description:We demonstrated recently that both constitutive and FAS-triggered apoptosis of human neutrophils are profoundly impaired by Francisella tularensis, but how this is achieved is largely unknown. To test the hypothesis that changes in neutrophil gene expression contribute to this phenotype, we used human oligonucleotide microarrays to identify differentially regulated genes in cells infected with F. tularensis strain LVS compared with uninfected controls. In order to examine the effect of F. tularensis on the neutrophil transcriptome, we performed microarray expression analysis on human neutrophils treated with F. tularensis subsp. holarctica live vaccine strain (LVS).

2012-10-22 | GSE37416 | GEO

Francisella tularensis subsp. holarctica LVS

Project description:Genome-wide analyses of PmrA from Francisella tularensis subsp. holarctica LVS

| PRJNA316710 | ENA

Gene expression data from F. tularensis-exposed neutrophils

2012-10-22 | E-GEOD-37416 | biostudies-arrayexpress

Transcriptomic responses to a live-attenuated Francisella tularensis vaccine

Project description:Impact of live attenuated F. tularensis vaccine (DVC-LVS) on PBMC poly(A)-RNA expresssion in 10 subjects over time (Days 1, 2 ,7, and 14 post-vaccination) relative to pre-vaccination (Day 0).

2020-06-30 | GSE149809 | GEO

Prior infection with Type A Francisella tularensis antagonizes the pulmonary transcriptional response to an aerosolized TLR4 agonist

Project description:Prior aerosol exposure to F. tularensis subsp. tularensis, but not the live attenuated strain (LVS) of F. tularensis subsp. holarctica or F. novicida, significantly antagonized the transcriptional response in the lungs of infected mice exposed to aerosolized TLR4 ligand E. coli LPS.

2015-09-30 | GSE65871 | GEO

Transcription profiling of Francisella tularensis LVS after heat shock

Project description:Effect of temperature upshift at 44°C for 30 minutes on transcription in Francisella tularensis LVS

2009-07-01 | E-MEXP-2037 | biostudies-arrayexpress

Comprehensive transposon insertion sequencing screen in Francisella tularensis LVS

Project description:We used transposon insertion sequencing (Tn-Seq) to identify the genes that are required for in vitro growth and intramacrophage growth of the live vaccine strain of F. tularensis (LVS).

2020-07-15 | GSE138658 | GEO

Characterization of F. tularensis LVS outer membrane vesicles

Project description:Francisella tularensis is a Gram-negative bacteria responsible for tularemia, a disease for which the high prevalence of failure and relapses is a main concern. Directed evolution experiments carried out in presence of antibiotics revealed that acquisition of fluoroquinolones (FQ) resistance was not exclusively related to mutations in DNA gyrase genes which are the first targets of these molecules. Here, using F. tularensis LVS as model, we investigated the role of FupA/B (Fer-Utilization Protein), whose possible contribution to FQ resistance was suggested by genomic analysis of resistant isolates. Using trans-complementation/mutagenesis approaches we definitely connected FupA/B expression to FQ sensitivity. Furthermore, we showed that the virulent strain F. tularensis subsp. tularensis SCHU S4, lacking the homologous FupA protein, exhibited a lower FQ susceptibility. Importantly, the deletion of this lipoprotein promoted an increased secretion of outer membrane vesicles (OMVs). Mass spectrometry-based quantitative proteomic characterization of OMVs from LVS and LVS-∆fupA/B strains led to the identification of 801 proteins among which a subset of 23 proteins exhibited a differential abundance between OMVs from both strains and may therefore contribute to the observed increased FQ susceptibility. We finally demonstrated that OMVs are structural key elements of F. tularensis LVS biofilms providing protection against FQ. Taken together, these results showed that mutations targeting FupA/B contribute to antimicrobial resistance through quantitative and qualitative modulations of OMV biogenesis and biofilm formation, providing new basis for understanding and fighting against Francisella antibiotic resistance and/or persistence.

2019-11-12 | PXD010305 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data