Project description:Gene expression in the obligatory aerobic acetic acid bacterium Gluconobacter oxydans was shown to respond to oxygen limitation, but the regulators involved are unknown. In this study, we analyzed the function of a transcriptional regulator named GoxR, which belongs to the FNR family. Here, we applied ChAP-seq analysis with a strep-tagged GoxR version to identify binding sites of this regulator in the genome of G. oxydans.
Project description:To identify CdbA binding sites on Myxococcus xanthus genome in vivo we performed ChIP-seq, using a polyclonal anti-FLAG antibody and a strain endogenously expressing CdbA_3xFLAG. A WT DK1622 strain was used a negative control and a strain endogenously expressing ParB_3xFLAG was used as positive control.