Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of alternative splicing under heat stress condition in atcyp18-1 mutant compared to wild type of Arabidopsis

ABSTRACT: Transcriptome analysis of alternative splicing under heat stress condition in atcyp18-1 mutant compared to wild type of Arabidopsis

PROVIDER: PRJNA670492 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transriptome analysis of alternative splicing under heat stress condition in atcyp18-2 mutant compared to Wild type (Col-0) of Arabidopsis

Project description:Transriptome analysis of alternative splicing under heat stress condition in atcyp18-2 mutant compared to Wild type (Col-0) of Arabidopsis

| PRJNA701911 | ENA

Transcriptome analysis of alternative splicing under heat stress condition in atcyp18-1 mutant compared to wild type of Arabidopsis

Project description:To investigate whether the observed changes in pre-mRNA splicing led to AtCYP18-1 depletion, we conducted RNA-sequencing to analyse the quantitative profiling of alternative splicing

2020-10-22 | GSE159796 | GEO

Transriptome analysis of alternative splicing under heat stress condition in atcyp18-2 mutant compared to Wild type (Col-0) of Arabidopsis

Project description:To investigate whether the observed changes in pre-mRNA splicing led to AtCYP18-2 depletion, we conducted RNA-sequencing to analyse the quantitative profiling of alternative splicing

2021-02-16 | GSE166755 | GEO

Heat stress induced alternative splicing in a species-dependent manner

Project description:Patterns of alternative splicing during heat stress in Arabidopsis thaliana and Boechera depauperata indicate complex and species-specific interactions between differential expression and alternative splicing.

2018-03-21 | GSE107820 | GEO

Alternative splicing in tomato pollen in response to heat stress

Project description:Alternative splicing caused by exposure of pollen to high temperatures leads to the generation of transcripts with pre-mature termination codon targeted for degradation or mRNAs putatively coding for truncated proteins with altered functions thereby controlling important cellular pathways including transport and localization, heat stress response, gene expression and various biosynthetic processes.

2016-11-04 | GSE80556 | GEO

Difference in gene expression between WT (Col-0) and atxrn4-3 mutant under non-stress and heat stress condition

Project description:Arabidopsis 5’-3’ exoribonuclease, AtXRN4, a homolog of yeast Xrn1p, functions in degradation of uncapped RNAs after de-capping step. While Xrn1p-dependent on plant XRN4’s targets for degradation is still limited. For understanding biological function of AtXRN4, we tested survivability of atxrn4 mutants under heat stress. Our results showed that atxrn4 mutants increased survival rate under short-term degradation is a main mRNA decay in yeast, knowledge heat stress compared with WT plants. Our microarray and mRNA decay assay showed that loss of AtXRN4 function caused reduction of mRNA degradation of heat shock factor A2 (HSFA2) and ethylene response factor 1 (ERF1). HSFA2 has been known as a key regulator in heat acclimation, was found as a target for AtXRN4 for degradation at non-stress condition. Heat stress applied on atxrn4-3 hsfa2 double mutant severely lacked heat tolerance phenotype of atxrn4 mutant. These results suggest that AtXRN4-mediated mRNA degradation linked to suppress heat acclimation.

2015-07-14 | GSE66369 | GEO

Cell type and culture condition-dependent alternative splicing in human breast cancer cells

Project description:We used splicing-sensitive microarrays to detect differences in alternative splicing between two breast cancer cell lines MCF7 (estrogen receptor positive) and MDA-MB-231 (estrogen receptor negative), as well as cultured human mammary epithelial cells (HMEC). Several splicing alterations in genes, including CD44, FAS, RBM9, HnRNPA/B, APLP2, and MYL6, were detected by the microarray, and verified by RT-PCR. We also compared splicing in these breast cancer cells cultured in either two-dimensional flat dishes (2-D) or in three-dimensional Matrigel (3-D) conditions. Only a subset of the splicing differences that distinguish MCF7 cells from MDA-MB-231 cells under 2-D culture condition is retained under 3-D conditions, suggesting that alternative splicing events are influenced by the geometry of the culture conditions of these cells. Keywords: Splicing-sensitive microarray

2005-12-30 | GSE3042 | GEO

Heat stress induced alternative splicing in a species-dependent manner

Project description:Heat stress induced alternative splicing in a species-dependent manner

| PRJNA421490 | ENA

Transformation of elongation factor 1Bdelta into heat shock response transcription factor by alternative splicing

Project description:Protein translation factors play crucial roles in a variety of stress responses. Here, we show that the eukaryotic elongation factor 1Bdelta (eEF1Bdelta) changes its structure and function from a translation factor into a heat shock response transcription factor by alternative splicing. While eEF1Bdelta is specifically localized in the cytoplasm, the long isoform of eEF1Bdelta (eEF1BdeltaL) is localized in the nucleus and induces heat shock element (HSE)-containing genes in cooperation with heat-shock transcription factor 1 (HSF1). Moreover, the N-terminal domain of eEF1BdeltaL binds with NF-E2-related factor 2 (Nrf2) and induces stress response heme oxygenase 1 (HO-1). Specific inhibition of eEF1BdeltaL with siRNA completely inhibits Nrf2-dependent HO-1 induction. In addition, eEF1BdeltaL directly binds to HSE oligo DNA in vitro and associates with HSE containing the HO-1-enhancer region in vivo. Thus, the transcriptional role of eEF1BdeltaL could provide new insights into the molecular mechanism of stress responses. We performed microarray analysis to compare the gene expression induced by eEF1Bdelta1 or eEF1BdeltaL overexpression. HEK293 cells transfected with expression plasmids encoding flag-tagged-eEF1Bdelta1 or eEF1BdeltaL protein

2011-04-12 | E-GEOD-28506 | biostudies-arrayexpress

Difference in gene expression between WT (Col-0) and atxrn4-3 mutant under non-stress and heat stress condition

2015-07-14 | E-GEOD-66369 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data