Blood-brain barrier alterations in human brain tumors revealed by genome-wide transcriptomic profiling
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA670696 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Brain tumors, either primary or secondary, have limited therapeutic options despite advances in understanding tumor driving gene mutations and heterogeneity within tumor cells. The cellular and molecular composition of brain tumor stroma, an important modifier of tumor growth, has been less investigated and understood. Especially, studies focusing on brain tumor blood vessels are rare, yet, the brain endothelium and the blood-brain barrier (BBB) are the major obstacle for efficient drug delivery. In this study we have employed the RNA sequencing approach to get insights into transcriptional alterations of endothelial cells isolated from primary and secondary brain tumors. We used an immunoprecipitation approach to enrich for endothelial cells of normal brain, glioblastoma (GBM) and adenocarcinoma brain metastasis (BM). Analysis of the endothelial transcriptome showed that both the GBM and the BM vasculature showed deregulation of genes implicated in cell proliferation, angiogenesis, deposition of extracellular matrix (ECM), and deregulation of genes defining the BBB dysfunction module. We describe alterations in the BBB genes in the GBM and BM vasculature and identify proteins that could be exploited for developing drug delivery platforms into primary and secondary brain tumors. In addition, we identify deregulated expression of genes defining vessel-associated fibroblasts in the GBM tissue, highlighting that the cellular composition of the brain tumor stroma deserves further investigation.
2021-02-10 | GSE159851 | GEO
Project description:Functional and structural dysfunction of the blood brain barrier (BBB) leads to severe alterations in brain physiology and is believed to trigger neurodegeneration. To investigate the molecular mechanisms driving the BBB dysfunction, very few human BBB cell culture models are available; of which, the human microvascular endothelial cell line (hCMEC/D3) is the most widely used. Thus far, array-based approaches or targeted seqeuncing based approaches have been employed to characterize the gene expression of the hCMEC/D3 model. However,The goal of this study is to perform deep transcriptomic sequencing of the BBB cell line and obtain features like gene expression, expressed single nucleotide variants, alternate splice forms, circular RNAs, long non-coding RNAs and micro RNAs. We have developed blood brain barriers transcriptomics landscape using RNA sequencing and micro RNA seqeuncing data obtained from replicates of hCMEC/D3 BBB cell line.
2016-03-17 | E-GEOD-76528 | biostudies-arrayexpress
Project description:Functional and structural dysfunction of the blood brain barrier (BBB) leads to severe alterations in brain physiology and is believed to trigger neurodegeneration. To investigate the molecular mechanisms driving the BBB dysfunction, very few human BBB cell culture models are available;of which, the human microvascular endothelial cell line (hCMEC/D3) is the most widely used. Thus far, array-based approaches or targeted seqeuncing based approaches have been employed to characterize the gene expression of the hCMEC/D3 model. However,The goal of this study is to perform deep transcriptomic sequencing of the BBB cell line and obtain features like gene expression, expressed single nucleotide variants, alternate splice forms, circular RNAs, long non-coding RNAs and micro RNAs. We have developed blood brain barriers transcriptomics landscape using RNA and micro RNA sequencing data obtained from replicates of hCMEC/D3 BBB cell line.
2016-03-17 | E-GEOD-76530 | biostudies-arrayexpress
Project description:BBBomics - Human Blood Brain Barrier Transcriptomics Hub
| PRJNA307647 | ENA
Project description:Human Blood Brain Barrier treated by Insulin
| PRJNA802135 | ENA
Project description:Differential analysis of plasma transmembrane proteins between two human blood-brain barrier model cells, hCMEC/D3 and HBMEC/cib cells.
2019-12-11 | PXD012021 | JPOST Repository
Project description:Functional and structural dysfunction of the blood brain barrier (BBB) leads to severe alterations in brain physiology and is believed to trigger neurodegeneration. To investigate the molecular mechanisms driving the BBB dysfunction, very few human BBB cell culture models are available;of which, the human microvascular endothelial cell line (hCMEC/D3) is the most widely used. Thus far, array-based approaches or targeted seqeuncing based approaches have been employed to characterize the gene expression of the hCMEC/D3 model. However,The goal of this study is to perform deep transcriptomic sequencing of the BBB cell line and obtain features like gene expression, expressed single nucleotide variants, alternate splice forms, circular RNAs, long non-coding RNAs and micro RNAs.
2016-03-17 | GSE76530 | GEO
Project description:Functional and structural dysfunction of the blood brain barrier (BBB) leads to severe alterations in brain physiology and is believed to trigger neurodegeneration. To investigate the molecular mechanisms driving the BBB dysfunction, very few human BBB cell culture models are available; of which, the human microvascular endothelial cell line (hCMEC/D3) is the most widely used. Thus far, array-based approaches or targeted seqeuncing based approaches have been employed to characterize the gene expression of the hCMEC/D3 model. However,The goal of this study is to perform deep transcriptomic sequencing of the BBB cell line and obtain features like gene expression, expressed single nucleotide variants, alternate splice forms, circular RNAs, long non-coding RNAs and micro RNAs.
2016-03-17 | GSE76528 | GEO
Project description:Using RNA sequencing to map differentially expressed genes in the 3D model of the blood-brain barrier ( composed of human brain endothelial cells, human astrocytes, and human pericytes) challenged with Borrelia garini .
2021-01-01 | E-MTAB-9753 | biostudies-arrayexpress
Project description:Using RNA sequencing to map differentially expressed genes in the 3D model of the blood-brain barrier ( composed of human brain endothelial cells, human astrocytes, and human pericytes) challenged with WNV.
2021-01-01 | E-MTAB-9754 | biostudies-arrayexpress