Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The Effect of Calcium on Activity, Immunogenicity and Efficacy of a Recombinant N1/N2 Neuraminidase Vaccine

ABSTRACT: The Effect of Calcium on Activity, Immunogenicity and Efficacy of a Recombinant N1/N2 Neuraminidase Vaccine

PROVIDER: PRJNA671601 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

The Effect of Calcium on Activity, Immunogenicity and Efficacy of a Recombinant N1/N2 Neuraminidase Vaccine

Project description:Despite the importance of immunity against neuraminidase (NA), NA content and immunogenicity is neglected in current influenza vaccines. To address this, a novel recombinant N1/N2 NA vaccine (NAV) was developed. Stability assays were used to determine optimal temperature and buffer conditions for vaccine storage. The effect of calcium-related enhancement of NA stability and activity on N1 and N2 immunogenicity and efficacy against viral challenge was assessed. Differences in activity between N1 and N2 and calcium-related activity enhancement did not translate into differences in immunogenicity or efficacy. NAV vaccinated mice showed robust antibody titers against N1 and N2, decreased viral titers, and decreased antiviral and inflammatory responses by transcriptomic analysis.

2021-03-30 | GSE160010 | GEO

The effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine.

Project description: Not available

| S-EPMC8024250 | biostudies-literature

Detoxins S1, N1, N2, N3, P1, P2, P3 source strain raw UHPLC-MS data and representative MS2

Project description:detoxin S1 source strain Streptomyces sp. S-325 (JOIW01) raw UHPLC-MS data and representative MS2 detoxins N1, N2, N3 source strain Streptomyces spectabilis NRRL 2792 raw UHPLC-MS data and representative MS2 detoxins N2, N3 source strain Streptomyces sp. NRRL B-1347 (JOJM01) raw UHPLC-MS data detoxins P1, P2, P3 source strain Amycolatopsis jujuensis NRRL B-24427 raw UHPLC-MS data and representative MS2

2019-04-01 | MSV000083648 | MassIVE

N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein products

Project description:The COVID mRNA vaccines utilize the modified nucleobase N1-methylpseudouridine, in place of canonical uridine, to improve immunogenicity and protein yield. However, relatively few studies have investigated the effect of modified nucleobases on the fidelity of protein translation. Given the interest in the COVID mRNA vaccines, we sought to investigate how N1-methylpseudouridine (and the related modification pseudouridine) is read by ribosomes.

2022-08-18 | PXD029291 | Pride

RNA-seq of mouse neutrophils polarized to proinflammatory N1 by lipopolysaccharide and interferon-γ or anti-inflammatory N2 by interleukin-4 versus control

Project description:Gene expression profiling for mouse N1 and N2 neutrophils. Neutrphils were isolated form the bone marrow of C57BL/6J mice between 12-16 weeks old. Approximately 8x10^7 neutrophils were used per condition and were split into 3 biological replicates. Freshly isolated neutrophils were cultured for 2h in RPMI medium, in the presence of 100 ng/ml lipopolysaccharide (LPS) and 20 ng/ml interferon gamma (IFNγ) or 20 ng/ml interleukin 4 (IL-4). Total RNA was isolated with TRIzol reagent and Phasemaker tubes. A sample from the LPS+IFNγ polarization did not pass the quality control test and was excluded from the downstream analysis.

2021-07-29 | E-MTAB-10508 | biostudies-arrayexpress

MAIT cell activation augments adenovirus vector vaccine immunogenicity

Project description:Mucosal-associated invariant T (MAIT) cells are innate sensors of viruses, which can augment early immune responses and contribute to protection from lethal infection. Thus, we reasoned MAIT cells may have an adjuvating role in the immunogenicity of replication-incompetent adenovirus vectors, which are novel vaccine platforms for pandemic pathogens such as Ebola virus and SARS-CoV-2. In both mice and human volunteers, immunization with ChAdOx1 (Chimpanzee Adenovirus Ox1) robustly activated MAIT cells. Activation required transduction of plasmacytoid dendritic cells and monocytes to produce IFN- and IL-18, respectively. IFN--induced monocyte-derived TNF was identified as a novel intermediate in this activation pathway, and activation required combinatorial signaling of all three cytokines both in vitro and in vivo. Strikingly, vaccine-induced activation of MAIT cells positively correlated with vaccine-induced T cell responses in human volunteers. Supporting a causal relationship, MAIT cell-deficient mice displayed impaired CD8+ T cell responses to multiple vaccine-encoded antigens. These findings define a novel role for MAIT cells in the immunogenicity of adenovirus vector vaccines, with potential implications for vaccine design.

2020-10-01 | GSE158835 | GEO

Escherichia coli N1 strain:N1

Project description:Escherichia coli N1 strain:N1 Genome sequencing

| PRJNA802380 | ENA

Nitrobacter sp. N1 strain:N1

Project description:Nitrobacter sp. N1 strain:N1 Genome sequencing

| PRJNA820293 | ENA

Altererythrobacter sp. N1 strain:N1

Project description:Altererythrobacter sp. N1 strain:N1 Genome sequencing

| PRJNA764449 | ENA

MAIT cell activation augments adenovirus vector vaccine immunogenicity

Project description:MAIT cell activation augments adenovirus vector vaccine immunogenicity