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RNA-seq analysis on SW480 cells treated with or without YH-620 under hypoxia

ABSTRACT: RNA-seq analysis on SW480 cells treated with or without YH-620 under hypoxia

PROVIDER: PRJNA673885 | ENA |

REPOSITORIES: ENA

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RNA-seq analysis on SW480 cells treated with or without YH-620 under hypoxia

Project description:To globally assess the role of alternatively translated 63 kDa NKRF, we performed and report on RNA-seq analysis on SW480 cells treated with or without novel small molecule HIF antagonist YH-620 under hypoxia. This study provides a framework for understanding the effect of novel small molecule HIF antagonist YH-620 on downstream targets. In addition, we also performed RNA-seq analysis on SW480 cells treated with or without specific small molecule CBP/catenin antagonist ICG-001 under hypoxia.

2021-06-30 | GSE160661 | GEO

RNA-seq of ITPR3 and RELB knockout in SW480 under normoxia and hypoxia

Project description:Here we compare the transcriptional profiles of SW480 cells with ITPR3 or RELB knockout with control cells under normoxia and hypoxia.

2022-09-20 | GSE197576 | GEO

Human colon cancer SW480 cells: stably transfected with vector control and LRP16 treated with or without etoposide

Project description:Transcriptional profiling of human colon cancer SW480 cells comparing control untreated SW480 cells with cells stably transfected with LRP16 treated with or without etoposide (50 μM) for the indicated periods (0,1hour, 3hours).An exploratory microarray analysis was performed with mRNA extracted from clutured SW480 cells transfected with LRP16 or control plasmid that were treated with or without etoposide. Total RNA of colon cancer cells stably transfected with vector control and LRP16 treated with or without etoposide (50 μM) for the indicated periods was isolated and purified using RNeasy Kit (Qiagen, Hilden, Germany). Integrity of RNA was assessed by using an Agilent BioAnalyser 2100 (Agilent Technologies).

2017-09-22 | GSE93625 | GEO

Rickettsia japonica YH strain:YH

Project description:Rickettsia japonica YH strain:YH Genome sequencing and assembly

| PRJNA175417 | ENA

Rickettsia japonica YH strain:YH

Project description:Rickettsia japonica YH genome sequencing

| PRJDA38487 | ENA

LncRNA expression analysis of Clostridium butyricum-induced cell apoptosis in colon cancer SW480 cells

Project description:To compare lncRNAs and mRNAs expression profiles in colon cancer after co-cultured with CB and without CB, we extracted total RNA of colon cancer cell line(SW480) after co-cultured with CB(SW480/CB) and paired control without CB(SW480/ctr), and identified the dysregulated lncRNAs and mRNAs using Agilent Human lncRNAs/mRNAs microarrays.

2015-05-30 | GSE66731 | GEO

Wnt signaling pathway gene expression profile in SW480 cells with/without hiNOS overexpression

Project description:Wnt signaling plays important roles in development and cancer. We found hiNOS is a wnt target gene. We tried to clarify the role of the so far iNOS in Wnt signaling in colorectal cancer. We performed SuperArray limited gene array analysis of SW480 cells with/without hiNOS overexpression. Oligo GEArray expression array systems (cat#OHS-043) SuperArray, Bethesda, MD) consisted of spotted cDNA fragments encoding 114 genes involved in and downstream of Wnt signaling. Control sequences (PUC18, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and ?-actin) were also included. These Superarrays were employed to compare wnt signaling genes expression between with iNOS and without iNOS. Total RNA was isolated by Trizol (Invitrogen) and processed for microarray hybridization following the manufacturer’s instructions. Arrays were visualized by autoradiography, and hybridization signals were scanned and analyzed for density in GEArray Expression Analysis Suite 2.0. The normalized value for each gene was calculated by dividing the value of each gene by the average value of the housekeeping genes GAPDH, and ?-actin. To obtain a gene profile, analysis was performed on three separate RNA samples isolated from different SW480 cells, with replicate assays conducted on each RNA preparation.

2013-08-20 | E-GEOD-50009 | biostudies-arrayexpress

Treatment of SW480 colon cancer cell induced xenografts with AZD and DBZ

Project description:RNASeq data from replicates with and without treatment of SW480 colon cancer cells

2018-04-18 | GSE98922 | GEO

ERbeta modulation of NFkB pathway in colon cancer cell lines SW480 and HT29

Project description:Analysis of differentially expressed genes in colon cancer cell lines SW480 and HT29 with and without stably expressed ERbeta gene, with and without 10ng/mL TNFa treatment for 2 and 24 hours. Total RNA obtained from colon cancer cell lines SW480 and HT29 with and without stably expressed ERbeta gene, with and without 10ng/mL TNFa treatment for 2 and 24 hours.

2016-07-17 | E-GEOD-65979 | biostudies-arrayexpress

ERbeta modulation of NFkB pathway in colon cancer cell lines SW480 and HT29

2020-08-13 | GSE65979 | GEO

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