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Transcriptome profiles of alternative MED19 LNCaP and control LNCaP cells cultured under androgen deprivation with vehicle or R1881

ABSTRACT: Transcriptome profiles of alternative MED19 LNCaP and control LNCaP cells cultured under androgen deprivation with vehicle or R1881

PROVIDER: PRJNA676100 | ENA |

REPOSITORIES: ENA

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Transcriptome profiles of alternative MED19 LNCaP and control LNCaP cells cultured under androgen deprivation with vehicle or R1881

Project description:We report the application of ChIP and RNA sequencing to identify the mechanism whereby stable overexpression of MED19 in androgen-dependent LNCaP cells promotes growth under conditions of androgen deprivation. We determined the MED19 and AR transcriptomes and cistromes in control and MED19 LNCaP cells. We also examined genome-wide H3K27 acetylation in both the absence and presence of androgens. We found that MED19 overexpression selectively alters AR occupancy, H3K27 acetylation, and gene expression. Under conditions of androgen deprivation, genes regulated by MED19 correspond to genes regulated by ELK1, a transcription factor that binds the AR N-terminus to induce select AR target gene expression and proliferation. This study provides important insight into the mechanisms of prostate cancer cell growth under low androgen, and underscores the importance of the MED19 in this process.

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Project description:LNCaP cells were grown in charcoal stripped media for 48hrs and then treated for 1hr with the synthetic androgen R1881 or ethanol (vehicle). Cell extracts from both treatment conditions were then subjected to chromatin-immunoprecipitation using an anti-androgen receptor antibody, before labeling and hybridisation to a microarray containing 24,000 gene promoter regions.

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Stable overexpression of MED19 in androgen-dependent LNCaP cells promotes growth under conditions of androgen deprivation

Project description:Stable overexpression of MED19 in androgen-dependent LNCaP cells promotes growth under conditions of androgen deprivation

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Identification of direct androgen-target genes in the prostate cancer cell line LNCaP

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