Algae metagenome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA677054 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Kaiparowitsia implicata GSE-PSE-MK54-09C and co-occurring heterotrophs from soil from Kaiparowits Plateau, Grand Staircase Escalante National Monument, UT, United states - 20190625_23
| PRJNA650883 | ENA
Project description:Pegethrix bostrychoides GSE-TBD4-15B and co-occurring heterotrophs from rock surface from Grand Staircase-escalante National Monument, Utah, United States - 20190225_38
| PRJNA617102 | ENA
Project description:Timaviella radians GSE-UNK-7R and co-occurring heterotrophs from rock surface from Grand Staircase Escalante National Monument, Utah, United States - 20181204_57
| PRJNA621672 | ENA
Project description:Timaviella obliquedivisa GSE-PSE-MK23-08B and co-occurring heterotrophs from rock surface from Grand Staircase Escalante National Monument, Utah, United States - 20181204_58
| PRJNA621673 | ENA
Project description:Aphanothece saxicola GSE-SYN-MK-01-06B and co-occurring heterotrophs from rock surface from Grand Staircase Escalante National Monument, Utah, United States - 20181031_5A
| PRJNA621658 | ENA
Project description:Cyanomargarita calcarea GSE-NOS-MK-12-04C and co-occurring heterotrophs from rock surface from Grand Staircase Escalante National Monument, Utah, United States - 20181105_13A
| PRJNA621661 | ENA
Project description:Tolypothrix brevis GSE-NOS-MK-07-07A and co-occurring heterotrophs from rock surface from Grand Staircase Escalante National Monument, Utah, United States - 20181105_59A
| PRJNA621662 | ENA
Project description:Aphanocapsa sp. GSE-SYN-MK-11-07L and co-occurring heterotrophs from rock surface from Grand Staircase Escalante National Monument, Utah, United States - 20181109_4A
| PRJNA621664 | ENA
Project description:The urea channel Slc14a2 (or UT-A1) mediates vasopressin-regulated urea transport across the inner medullary collecting duct (IMCD). Previously, UT-A1 was found to present in a high molecular weight complex, suggesting UT-A1 is involved in certain protein-protein interactions. The present study sought to identify the proteins that interact with UT-A1 in this complex for a better understanding of how UT-A1 is regulated. Rat IMCD suspensions were treated with or without V2 receptor agonist, dDAVP, followed by in-cell crosslinking using BSOCOES and detergent solubilization. Immunoprecipitation using Dynabeads coated with UT-A1 specific antibody successfully pulled down the UT-A1 proteins. In-gel digestion protocol was carried out to prepare samples for liquid chromatographic mass spectrometry analysis of tryptic peptides using a Velos-Orbitrap mass spectrometer. The peptides passing stringent spectral quality thresholds were quantified (label-free) to identify those with (UTA-1 antibody/preimmune IgG) >4. A total of 128 UT-A1 interacting proteins were identified. Gene Ontology analysis maps the distribution of these proteins throughout major cell compartments: endoplasmic reticulum, Golgi, endosomes, cytosol and plasma membrane. Among them are four protein kinases (Cdc42bpb, Phkb, Camk2d, Mtor) that play roles in vasopressin-regulated phosphorylation of UT-A1. Non-label quantification was also performed to determine the stoichiometry of UT-A3 with UT-A1, the result does not support an oligomeric complex formation of UT-A1/A3. In conclusion, we have provided a refined list of UT-A1 binding proteins which can be useful for further analysis of the vasopressin signaling pathway in regulation of UT-A1 in IMCD.
2019-11-08 | PXD003496 | Pride
Project description:We sampled lake-type and riverine sockeye in the pristine natural habitats of Aniakchak National Monument and Preserve, Alaska USA.
2011-09-10 | GSE31214 | GEO