Project description:This study investigates the impact of Dexamethasone on sperm RNA in mice. Using RNA sequencing we find that Dexamethasone impacts the sperm payload significantly 2 weeks post intra peritoneal administration. Sperm RNA was sequenced 14 days following 2mg/kg dexamethasone or vehicle intraperitoneal injection.
Project description:2-cell embryo RNA was sequenced following IVF of naive untreated oocytes with sperm harvested 2 weeks after 2mg/kg dexamethasone or vehicle intraperitoneal injection in males.
Project description:In order to discover novel small RNAs expressed in mature sperm, we isolated mature sperm from mouse cauda epididymis, comparing with data from adult tesis and uterus. The small RNA fraction (18-40nt) was cloned and sequenced from total RNA of mature sperm, testis and uterus of mice. RNAs extracted from mature sperm, adult testis and uterus were used for high throughput sequencing analysis