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Vascular integrity is essential for maintaining tissue homeostasis I

ABSTRACT: Vascular integrity is essential for maintaining tissue homeostasis I

PROVIDER: PRJNA681529 | ENA |

REPOSITORIES: ENA

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Vascular integrity is essential for maintaining tissue homeostasis

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Project description:Purpose: analyze the transcriptomic difference of endothelial cells and pericytes in PBS, LPS or LPS + Riociguat treated lungs Methods: Gucy1a1-EGFP mice were given PBS or LPS treatment via trachea instillation. Vehicle or Riociguat oral administation was performed at every 9 hours. 24h later, lungs were dissociated and endothelial cells and pericytes were isolated by flow cytometry, then directly processed to RNA extraction and library construction Results: Riociguat can restore LPS-induced lung injury by inhibiting pericytes activation and maintain vascular homostatsis

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Project description:Purpose: analyze the transcriptomic differences in PBS and Riociguat treated whole lung lysate Methods: C57BL6 mice were given PBS or Riociguat treatment (i.g.). 24h later, homogenize lung and extract mRNA Results: Riociguat has merely effect on normal mice lung

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Epithelial EP4 plays an essential role in maintaining homeostasis in colon

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Vascular integrity is essential for maintaining tissue homeostasis I

Project description:Purpose: analyze the transcriptomic differences in PBS, LPS and LPS + Riociguat treated whole lung lysate Methods: C57BL6 mice were given PBS, LPS or LPS + Riociguat treatment via trachea instillation. 24h later, homogenize lung and extract mRNA Results: Riociguat can restore LPS-induced lung injury by improving immune environment

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