Project description:Purpose:The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) of different resistance to sheath blight in rice chromosome segment substitution lines
Project description:rice flag leaves at heading stage from three chromosome substitution line populations, which were respectively constructed by introducing genomic segments from japonica cultivar Niponbare, indica cultivar Minghui 63 and wild accession ACC10, to an indica cultivar Zhenshan 97, were collected. Metabolomics profile was conducted to generate quantitative trait loci that may affect contents of metabolites, and candidate genes were assigned.
2018-08-06 | ST001031 | MetabolomicsWorkbench
Project description:Transcriptome data of cotton chromosome segment substitution line
| PRJNA514818 | ENA
Project description:Rice Height QTL in KDML105 Chromosome Segment Substitution Lines
Project description:Improvement of chilling tolerance is a key strategy to face potential menace from abnormal temperature in rice production, which depends on the signaling network triggered by receptors. However, little is known about the QTL genes encoding membrane complexes for sensing cold. Here, Chilling-tolerance in Gengdao/japonica rice 1 (COG1) was isolated from a chromosome segment substitution line containing a QTL (qCS11-jap) for chilling sensitivity. The major gene COG1 was found to confer chilling tolerance in japonica rice. In natural rice populations, only the haplogroup1 encoded a functional COG1. Evolutionary analysis showed that COG1 originated from Chinese O. Rufipogon and was fixed in japonica rice during domestication. COG1, a membrane-localized LRR-RLP, targeted and activated the kinase OsSERL2 in a cold-induced manner, promoting chilling tolerance. Furthermore, the cold signal transmitted by COG1-OsSERL2 activates OsMAPK3 in the cytoplasm. Our findings reveal a cold-sensing complex, which mediates signaling network for the chilling defense in rice.
Project description:<p>The common cutworm (CCW; <em>Spodoptera litura</em>) is one of the major insect pests of soybean in Asia and Oceania. Although quantitative trail loci related to CCW resistance have been introduced into leading soybean cultivars, these do not exhibit sufficient resistance against CCW. Thus, understanding the genetic and metabolic resistance mechanisms of CCW as well as integrating other new resistance genes are required. In this study, we focused on a primitive soybean landrace, Peking, which has retained resistances to various pests. We found a resistance to CCW in Peking by the detached-leaf feeding assay, and subsequently determined the genetic and metabolic basis of the resistance mechanism using chromosome segment substitution lines (CSSLs) of Peking. Several characteristic metabolites for Peking were identified by the metabolomic approach using liquid chromatography/mass spectrometry combined with a principle component analysis. The structure of seven metabolites were determined by nuclear magnetic resonance (NMR) analysis. The genomic segments of Peking on chromosome 06 (Chr06) and Chr20 had a clear association with these metabolites. Moreover, a line possessing a Peking genomic segment on Chr20 inhibited growth of the CCW. The genetic factors and the metabolites on Chr20 in Peking will be useful for understanding mechanisms underlying CCW resistance and breeding resistant soybean cultivars.</p>
2020-11-06 | MTBLS1960 | MetaboLights
Project description:Genomic resequencing of 3 sets of rice chromosome segment substitution lines.
Project description:To investigate mouse intersubspecific divergence of transcriptional regulation between C57BL/6J (B6) and Japanese wild-derived MSM/Ms strains, we performed transcriptome analysis by microarray on liver from B6 and MSM, and B6-ChrNMSM chromosome substitution strain panel, which carries MSM-derived chromosome or chromosomal segment on the B6 host.
Project description:Transcriptome analysis of axillary meristems of rice cultivar Takanari and a near isogenic line that have a chromosomal segment from rice cultivar Habataki.
