Project description:Understanding the coordinated regulation of the hundreds of carbohydrate-active enzyme (CAZyme) genes occurring in the genomes of fungi has great practical importance. We recorded genome-wide transcriptional changes of Aspergillus nidulans cultivated on glucose, lactose or arabinogalactan as well as under carbon starved conditions. We determined both carbon stress specific changes (a carbon stress vs. glucose) and culture specific changes (a culture vs. all other cultures). Many CAZyme genes showed carbon stress specific and/or culture specific upregulation on arabinogalactan (138 and 62 genes, respectively). Besides galactosidase and arabinan degrading enzyme genes, enrichment of cellulolytic, pectinolytic, mannan and xylan degrading enzyme genes were observed in these gene sets. Less, 81 and 107 carbon stress specific as well as 6 and 16 culture specific upregulated genes were found on lactose and in carbon starved cultures, respectively. They were enriched only in galactosidase and xylosidase genes on lactose and rhamnogalacturonanase genes in both cultures. Some CAZyme genes (29 genes) showed culture specific upregulation on glucose and they were enriched in beta-1,4-glucanase genes. Behavioral ecological background of these characteristics was evaluated to organize comprehensively our knowledge on CAZyme production, which can lead to develop new strategies to produce enzymes for plant cell wall saccharification.
