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Genome wide map of chromatin state during early development of TrkC neurons of the DRG

ABSTRACT: Genome wide map of chromatin state during early development of TrkC neurons of the DRG

PROVIDER: PRJNA681913 | ENA |

REPOSITORIES: ENA

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Genome wide map of chromatin state during early development of TrkC neurons of the DRG

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Comparative microarray analysis of NaV1.8-expressing DRG neurons and cold-sensitive non-NaV1.8-expressing DRG neurons

Project description:The vast majority of cold sensitive DRG neurons from mice do not express the voltage-gated sodium channel NaV1.8. Therefore, we aimed to compare the molecular profiles of NaV1.8 and non-NaV1.8-expressing neurons using microarray analysis. Fluorescent activated cell sorting was performed at 4 degrees centigrade on acutely dissociated DRG neurons from mice expressing NaV1.8-Cre, Pirt-GCaMP3 and a Cre-dependent global reporter (td tomato). NaV1.8-expressing neurons were sorted based on their reporter fluorescence (td tomato; red) and putative cold sensing neurons were sorted based on their GCaMP3 fluorescence at 4 degrees centigrade and absence of Cre-dependent reporter fluorescence. A total of three mice were used (samples one, two and three) with GCaMP3 only and NaV1.8-expressing neurons forming two relative populations within each sample (eg. GC3 one is the experimental counterpart of Tom one).

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Gene expression profile in DRG neurons over-expressing NRG1 type III intracellular domain

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RNA sequencing of whole Dorsal Root Ganglia (DRG) and laser captured large diameter DRG neurons upon Environmental Enrichment (EE) compared to Standard Housing (SH)

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