Project description:We measured protein translation (by ribosome profiling) and RNA levels (by polyA-enriched RNA-seq) in Cryptococcus neoformans strain H99 and Cryptococcus neoformans strain JEC21. This is the first transcriptome-wide map of translation in this species complex.
Project description:We investigated the effects of the hypoxia-mimetic CoCl2 on the gene expression of pathogenic fungus Cryptococcus neoformans. Keywords: compound treatment design
Project description:Mating pheromone is the best-known fungal paracrine signal whose essentiality in determining sexual reproduction has been recognized since six decades ago. We demonstrate that the pheromone cannot induce mating response during unisexual mating due to the lack of a compatible receptor from the same unisexual population. We identify a quorum sensing (QS) peptide Qsp1 in place of pheromone as a specific intercellular signal directing Cryptococcus unisexual reproduction. A typical zinc finger regulator Cqs2 as the master regulator of QS signaling induces unisexual reproduction in response to Qsp1. Collectively, these data demonstrate that QS activation rather than pheromone induction as the pivotal social control mechanism underlies unisexual reproduction in C .neoformans.
Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 4ug/ml benomyl. Randomly 60 adaptors were sequenced.
Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 2.5ug/ml chlorothalonil. Randomly 72 adaptors were sequenced.
Project description:Cryptococcus neoformans interactions with murine macrophages are critical for disease. In this project we analyzed fungal proteins which were co-purified with murine host proteins after interaction. H99 C. neoformans was opsonized with mAb 18B7 and addedd to murine macrophages. Then murine cells were lysed and cell extracts submitted to proteomics.
Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 1.5ug/ml pyraclostrobin. Randomly 23 adaptors were chosen. These adaptors were sequenced.
Project description:Cryptococcus neoformans lab strain H99 was exposed to 600ng/ml aureobasidin A. Randomly 30 adaptors were chosen. These adaptors and the wild type strains were sequenced.
