Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Molecular diversity of olfactory bulb projection neurons [single-cell RNA-seq]

ABSTRACT: Molecular diversity of olfactory bulb projection neurons [single-cell RNA-seq]

PROVIDER: PRJNA682579 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Molecular diversity of olfactory bulb projection neurons

Project description:Molecular diversity of olfactory bulb projection neurons

| PRJNA682577 | ENA

Molecular diversity of olfactory bulb projection neurons [single-cell RNA-seq]

Project description:To characterize the molecular diversity of olfactory bulb projection neurons we used viral targeting and Fluorescence Activated Nuclei Sorting (FANS) to enrich for olfactory bulb projection neurons, and single-nuclei RNA sequencing (sn-RNA seq) to comprehensively characterize their transcriptomes. To isolate GFP-labelled nuclei, 3 individual replicates of AON and PCx-injected mice were used. Ipsilateral and controlateral sides were minced separately and placed into two different tubes. The minced tissue was gently homogenized in Nuclei PURE Lysis Buffer and 10% Triton X-100 using an ice-cold dounce and pestle, and filtered two times through a 40 μm cell strainer on ice. After centrifuging at 500 rpm for 5 min at 4 °C, the supernatant was aspirated and gently resuspended in 500 μl of cold buffer (1x of cold Hanks' Balanced Salt Solution HBSS, 1% nuclease-free BSA, RNasin Plus and 1/2000 DRAQ5). Our study identifies molecularly distinct subtypes of mitral and tufted cells.

2020-12-05 | GSE162654 | GEO

Retrograde labelling and RNA deep sequencing of olfactory bulb projection neurons [bulk RNA-seq]

2020-12-05 | GSE162655 | GEO

Validation of molecularly-defined olfactory bulb projection neurons targeting anterior or posterior brain areas

Project description:To validate different projection targets of already molecularly-defined olfactory bulb projection neurons we used viral targeting specifically into anterior or posterior cortical areas, Fluorescence Activated Nuclei Sorting (FANS) to enrich for olfactory bulb projection neurons, and single-nuclei RNA sequencing (sn-RNA seq) To isolate GFP-labelled nuclei, 1 individual replicate of AON or PCx-injected mice was used. Ipsilateral and controlateral sides were minced separately and placed into two different tubes. The minced tissue was gently homogenized in Nuclei PURE Lysis Buffer and 10% Triton X-100 using an ice-cold dounce and pestle, and filtered two times through a 40 μm cell strainer on ice. After centrifuging at 500 rpm for 5 min at 4 °C, the supernatant was aspirated and gently resuspended in 500 μl of cold buffer (1x of cold Hanks' Balanced Salt Solution HBSS, 1% nuclease-free BSA, RNasin Plus and 1/2000 DRAQ5). Our study identifies molecularly distinct subtypes of mitral cells projecting to anterior or posterior olfactory cortices.

2021-06-09 | GSE176350 | GEO

Pathological consequences of chronic olfactory inflammation on neurite morphology of olfactory bulb projection neurons

Project description:Pathological consequences of chronic olfactory inflammation on neurite morphology of olfactory bulb projection neurons

| PRJNA771410 | ENA

RNA sequencing of olfactory bulb projection neurons in neurotensin-GFP (NTS-GFP) mice at different developing time points

Project description:Characterize the spatiotemporal dynamics of gene expression in neurons in developing olfactory bulb

2016-04-15 | GSE70896 | GEO

Pathological consequences of chronic olfactory inflammation on neurite morphology of olfactory bulb projection neurons

Project description:The effects of chronic olfactory inflammation on the olfactory bulb (OB) were examined. Inflammation was induced in the olfactory epithelium by repeated unilateral intranasal administration of lipopolysaccharide (LPS). After 4 weeks, gene expression profiles were compared between ipislateral and contralateral OBs using RNA sequencing analysis.

2022-04-06 | GSE185945 | GEO

Mouse olfactory sensory neurons, main olfactory epithelium and main olfactory bulb

Project description:Mouse olfactory sensory neurons, main olfactory epithelium and main olfactory bulb

| PRJNA714906 | ENA

Single-cell RNA sequencing of olfactory bulb dopaminergic neurons

Project description:Here we performed single cell RNA sequencing of manually sorted olfactory bulb dopaminergic neurons with the goal of studying the transcriptomic basis of neuronal plasticity. Acute olfactory bulb 300 µm slices were obtained from Dat-Cre/Flox-tdTomato (B6.SJL-Slc6a3tm1.1(cre) Bkmn/J, Jax stock 006660 / B6.Cg– Gt(ROSA)26Sortm9(CAG-tdTomato)Hze, Jax stock 007909) juvenile mice. Single cell suspensions were generated using the Neural Tissue Dissociation Kit – Postnatal Neurons (Miltenyi Biotec. Cat no. 130-094-802). Dopaminergic neurons were identified by red fluorescence and manually sorted into individual wells. Analysis of the effects of the transcriptomic changes relating to neuronal plasticity triggered by sensory deprivation is ongoing.

2020-06-15 | GSE151709 | GEO

Molecular diversity of olfactory bulb projection neurons

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-05 | GSE162656 | GEO