Project description:This project generated a high-coverage WGS dataset of 1,000+ highly admixed elderly from a census-based cohort from São Paulo, Brazil (SABE Study). Current Brazilian population results from admixture of Iberian Europeans, West and East Africans and Native Americans, and from 19th century onwards other groups from Europe, Asian and Middle East arrived in Brazil. The phase 1 includes BAM files from 1,171 individuals, described in pre-print "Whole-genome sequencing of 1,171 elderly admixed individuals from the largest Latin American metropolis (São Paulo, Brazil)" (DOI: 10.1101/2020.09.15.298026).
Project description:In the 15th century, approximately 900,000 Native Americans, mostly TupÃÂ-speakers, lived on the Brazilian coast. By the end of the 18th century, the coastal native populations were declared extinct. The Tupàarrived on the east coast after leaving the Amazonian basin approximately 2,000 years before present; however, there is no consensus on how this migration occurred - toward the northern Amazon and then directly to the Atlantic coast, or heading south into the continent, and then migrating to the coast. Here we leveraged genomic data from one of the last remaining putative representatives of the Tupàcoastal branch, a small, admixed, self-reported Tupiniquim community, as well as data of a GuaranàMbyá native population from Southern Brazil and of three other native populations from the Amazonian region. We demonstrated that the Tupiniquim Native American ancestry is not related to any extant Brazilian Native American population already studied and thus they could be considered the only living representatives of the extinct Tupàbranch that used to settle the Atlantic Coast of Brazil. Furthermore, these data show evidence of a direct migration from Amazon to the Northeast Coast in pre-Columbian time, giving rise to the TupàCoastal populations, and a single distinct migration southward that originated the Guaranàpeople from Brazil and Paraguay. This is the first study to elucidate the population dynamics and diversification of the Brazilian Natives at a genomic level, which was made possible by recovering data from the Brazilian coastal population through the genomes of mestizo individuals.
Project description:Human oocyte cDNA library was hybridized on a multi-species oocyte array (Bovine, Mouse, Frog) Temperature stringency criteria was used to evaluate the conservation degree of oocyte genes among vertebrates (Bovine, Mouse, Frog)
