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The effect of Methionine Restriction using oxidized casein as protein source on the transcriptome of mouse IWAT

ABSTRACT: The effect of Methionine Restriction using oxidized casein as protein source on the transcriptome of mouse IWAT

PROVIDER: PRJNA683810 | ENA |

REPOSITORIES: ENA

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The effect of Methionine Restriction using oxidized casein as protein source on the transcriptome of mouse IWAT

Project description:Ingestion of a diet with a restricted Methionine (MR) content results in robust activation of a set of browning genes in IWAT (inguinal white adipose tissue). So far, data were obtained using a mix of elemental amino acids as the dietary protein source, which was replaced in this experiment with casein. To reduce methionine content, casein was oxidized and methionine was added back to a concentration similar to the diets using elemental amino acids as protein source. Results show that mice reduce body weight and adiposity on MR containing oxidized casein similar to MR containing elemental amino acids. Similarly, energy expenditure, as well as food and water intake were increased. The RNAseq experiment was conducted to compare the effect of MR using these two protein sources on the IWAT transcriptome.

2021-06-09 | GSE162917 | GEO

The effect of Methionine Restriction using oxidized casein as protein source on the transcriptome of mouse livers

Project description:Ingestion of a diet with a restricted Methionine (MR) content results in robust activation of hepatic stress pathways regulated by PERK, ATF4, and Nfe2l2. In addition, genes regulating hepatic lipogenesis are downregulated. So far, data were obtained using a mix of elemental amino acids as the dietary protein source, which was replaced in this experiment with casein. To reduce methionine content, casein was oxidized and methionine was added back to a concentration similar to the diets using elemental amino acids as protein source. Results show that mice reduce body weight and adiposity on MR containing oxidized casein similar to MR containing elemental amino acids. Similarly, energy expenditure, as well as food and water intake were increased. The RNAseq experiment was conducted to compare the effect of MR using these two protein sources on the hepatic transcriptome.

2021-06-09 | GSE162918 | GEO

The effect of Methionine Restriction using oxidized casein as protein source on the transcriptome of mouse livers

Project description:The effect of Methionine Restriction using oxidized casein as protein source on the transcriptome of mouse livers

| PRJNA683811 | ENA

Effects of 20 months Dietary Methionine Restriction on Gene Expression in inguinal white adipose tissue of Fischer 344 Rats

Project description:Dietary methionine restriction (MR) has been shown to increase lifespan and decrease adiposity in rodents. This study was designed to examine the transcriptional effects of MR in metabolically relevant tissues. This experiment contains data from the inguinal white adipose tissue (IWAT). We analyzed MR-induced changes in gene expression using pooled RNA from IWAT of rats fed either a control purified amino acid diet (DL-methionine content of 0.86%) (CON) or a methionine-restricted diet (DL-methionine content of 0.172%)(MR). Rats were fed Purina rodent diet 5001 until 32 days of age and were then randomly assigned to be fed CON diet or MR diet for 20 months.

2013-07-01 | E-GEOD-28599 | biostudies-arrayexpress

Effects of 20 months Dietary Methionine Restriction on Gene Expression in inguinal white adipose tissue of Fischer 344 Rats

2013-07-01 | GSE28599 | GEO

The effect of dietary methionine restriction using different protein sources on the transcriptome of mouse livers

Project description:Dietary Methionine restriction (MR) has been demonstrated to induce a set of hepatic responses, including activation of hepatic stress response and suppression of hepatic lipogenesis. In the current study, we used two different protein sources to achieve MR. Casein was used in normal protein (NP, 20% casein) and low protein (LP, 5% casein) diet. Soy protein was used in 20Soy (20% soy), 20Soy+SAA (20% soy, Met and Cys was added back to match their concentration in NP diet), and 10Soy (11.5% soy, the Met concentration matches LP diet). Results show that mice that were fed LP and 10Soy diet have lower body weight and adiposity and increased food and water intake, energy expenditure, and serum FGF21 levels compared to their relative controls. The RNAseq experiment was conducted to compare the effect of MR using these two protein sources on the hepatic transcriptome.

2021-09-01 | GSE168132 | GEO

scRNA-seq of aged Drosophila midgut under methionine restriction

Project description:Methionine restriction is known to extend lifespan in various model organisms including Drosophila melanogaster. In this analysis, we performed scRNAseq of Drosophila female midgut samples to understand the cell type specific response to methionine restriction.

2023-09-25 | GSE198149 | GEO

Effects of 20 months Dietary Methionine Restriction on Hepatic Gene Expression in Fischer 344 Rats

Project description:Dietary methionine restriction (MR) has been shown to increase lifespan and decrease adiposity in rodents. This study was designed to examine the transcriptional effects of MR in metabolically relevant tissues. This experiment contains data from the liver. We analyzed MR-induced changes in gene expression using pooled RNA from liver of rats fed either a control purified amino acid diet (DL-methionine content of 0.86%) (CON) or a methionine-restricted diet (DL-methionine content of 0.172%)(MR). Rats were fed Purina rodent diet 5001 until 32 days of age and were then randomly assigned to be fed CON diet or MR diet for 20 months.

2012-04-07 | E-GEOD-28482 | biostudies-arrayexpress

Dietary methionine restriction impairs anti-tumor immunity through gut microbiota

Project description:Dietary methionine restriction represses growth and improves therapeutic responses in several pre-clinical settings. However, how this dietary intervention impacts cancer progression in the context of the immune system is unknown. Here we analyzed the CD45+ immune cells from the small intestine of control (CTRL) diet or methionine-restricted (MR) diet fed tumor-free C57BL/6J donor mice and tumor-bearing Apc <min+/-> recipient mice transplanated with feces from these diet-fed tumor-free C57BL/6J mice by scRNA-seq. Our analysis indicate that fecal microbes from methionine-restricted tumor-free C57BL/6J mice are sufficient to represss T cell activation in the small intestine of Apc <min+/-> mice.

2023-06-14 | GSE181220 | GEO

Oxidized methionine melanoma epitopes

Project description:One of the most promising alternatives to chemotherapy in cancer treatment is immunotherapy, particularly, peptide-based immunotherapy in which synthetic peptides derived from tumor-specific antigens are used to trigger an immune response to specifically target cancer cells. Here using custom nanoLC-MS/MS based workflows we examine the possible presentation of peptide-based oxidative variants in complex with Major Histocompatibility Complex (MHC) class I proteins at the surface of melanoma cells. The deposited data contains the raw files associated with mild acidic elutions (MAE) of MHC class I associated peptides from various melanoma cell lines. These were analysed using Data Independent Acquisition (DIA), employing a custom method for MS/MS fragmentation of the native and Met-oxidative modifications of a tyrosinase derived peptide (YMDGTMSQV denoted as YMD peptide). The data contains also the raw files referring to the nanoLC-MS/MS analysis of HPLC purified fractions of various oxidative derivatives of the YMD peptide but also of MAGE-10 native and oxidized forms.

2023-07-25 | PXD039318 | Pride

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